Morphological features of the transgenic tobacco plant shoot expressing the 3-hydroxy-3-methylglutagyl-CoA reductase (HMG1) gene in the direct and reverse orientations towards the promoter / Ermoshin A. A.,Kiseleva I. S.,Bortsova S. A.,Sanaeva Y. V.,Alekseeva V. V. // RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY. - 2016. - V. 47, l. 4. - P. 216-222.

ISSN/EISSN:

1062-3604 / 1608-3326

Type:

Article

Abstract:

3-Hydroxy-3-methylglutaryl-CoA reductase (HMG1) catalyzes the formation of mevalonic acid, the key intermediate of the cytosolic isoprenoid synthesis pathway. The parameters of stem and leaf growth were studied in the transgenic tobacco plants that express the HMG1 gene in both sense and antisense orientations towards the constitutive promoter. The transgenic plant height did not significantly differ from that of the control plants, though the plants carrying the sense copy of the HMG1 gene were considerably taller than plants that carried the antisense gene copy. Plants carrying an extra copy of the HMG1 gene were also characterized by increased leaf area. The number of mesophyll cells calculated per square unit of transgenic plants leaves was smaller than in the control plant leaves, though their volume was not considerably changed in any of the variants, suggesting changes in the cell packing density in leaves.

Author keywords:

transgenic tobacco plants; HMG1 gene; shoot height; leaf area; mesophyll COA REDUCTASE; BIOSYNTHESIS; ARABIDOPSIS; GROWTH; PATHWAY; ENZYME; DNA

DOI:

10.1134/S1062360416040044

Web of Science ID:

ISI:000381213400007

Соавторы в МНС:

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Другие поля

Поле	Значение
Month	JUL
Publisher	MAIK NAUKA/INTERPERIODICA/SPRINGER
Address	233 SPRING ST, NEW YORK, NY 10013-1578 USA
Language	English
EISSN	1608-3326
Keywords-Plus	COA REDUCTASE; BIOSYNTHESIS; ARABIDOPSIS; GROWTH; PATHWAY; ENZYME; DNA
Research-Areas	Developmental Biology
Web-of-Science-Categories	Developmental Biology
Author-Email	bos\_o@mail.ru
ResearcherID-Numbers	Ermoshin, Alexander/N-3613-2016
ORCID-Numbers	Ermoshin, Alexander/0000-0003-2784-4898
Funding-Acknowledgement	Ministry of Education and Science of the Russian Federation (Program Aimed at the Increasing of Competitiveness of Ural Federal University); Russian Foundation for Basic Researches {[}15-04-08380]
Funding-Text	This work was partly supported by the Ministry of Education and Science of the Russian Federation (Program Aimed at the Increasing of Competitiveness of Ural Federal University) and by the Russian Foundation for Basic Researches (project no. 15-04-08380)
Number-of-Cited-References	30
Usage-Count-Last-180-days	3
Usage-Count-Since-2013	3
Journal-ISO	Russ. J. Dev. Biol.
Doc-Delivery-Number	DT1AF