Characterization of chitin and its complexes extracted from natural raw sources / Kovaleva E., Pestov A., Stepanova D., Molochnikov L. // AIP Conference Proceedings. - 2016. - V. 1772, l. .

ISSN:

0094243X

Type:

Conference Paper

Abstract:

It is known that the main source of chitin and chitosan are shells of shrimp and other sea crustaceans. Alternative row sources of chitin, chitosan and its complexes are the lowest plants - mushrooms and insects. Also industrial wastes, especially from brewing of beer and manufacture of wine and ethanol can be used for extracting chitosan-containing products. The present research is aimed to the extraction of chitin and its complexes from alternative row sources, such as insects (cockroaches Pariplaneta Americana linnaeus and bees Apis mellifera lineaus), mushrooms (Amanita phalloides and Lactarius subdulius), waste banana wine (Kovibar and Urwibutso Inc.) and beer products (Bralirwa Inc., traditional sorghum) characteristic for Rwanda and their characterization using FTIR spectroscopy and elementary analysis. In chitin and its complexes extraction from all used raw sources, conditions for deproteinization were: 8% NaOH at 95 °C for 1 h and demineralization involved treatment with 6.7 % HCl at room temperature. Chitin and its complexes in the extracted samples were identified by FTIR spectroscopy using reference sample of Aspegillus Niger mushrooms. The presence of chitin parts causes the absorption band at 1650, 1552 and 1376 cm-1, which correspond to vibrations of amide groups amide I amide II and amide III, respectively. Using elemental analysis, the ratios of chitin and glucan parts were estimated and the percentage of chitin composition of all species was determined. For most of raw sources a fraction of chitin part was greater than that of glucan part. The chitin content of the samples studied ranged between 0.7-0.8 % of DM (dried mass) for wine (beer) waste products and 38% of dried mass (DM) for cockroaches. © 2016 Author(s).

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Index keywords:

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DOI:

10.1063/1.4964577

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https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994181887&doi=10.1063%2f1.4964577&partnerID=40&md5=eea3f9a62d4111791dd0f14c677a51f1

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Поле	Значение
Art. No.	050007
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994181887&doi=10.1063%2f1.4964577&partnerID=40&md5=eea3f9a62d4111791dd0f14c677a51f1
Affiliations	Ural Federal University, 19 Mira St., Yekaterinburg, Russian Federation; I.Ya. Postovsky Institute of Organic Synthesis, Ural Branch of the Russian Academy of Sciences, Akademicheskaya/S. Kovalevskoi, 22/20, Ekaterinburg, Russian Federation; Ural State Forest Engineering University, 37 Siberian Highway, Yekaterinburg, Russian Federation
Funding Details	14-03-00898, RFBR, Russian Foundation for Basic Research
Funding Text	This work was supported by the Russian Foundation for Basic Research (Grant 14-03-00898), the Program 211 of the Government of the Russian Federation No 02.A03.21.0006 and the State Tasks of the Ministry of Education (Russian Federation) No. 4.1626.2014/K and No. 2014/239 and the local Grant of University of Rwanda "Chitosancontaining materials of multifunctional application for needs of Rwanda". The authors are thankful to J.P. Intwali (Rwanda), E.V. Habumugisha (Rwanda), Ch. Ukundineza (Rwanda) and D. Niyoyita (Rwanda) for capturing insects and gathering mushrooms and their initial preparation for chitin extraction.
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Correspondence Address	Kovaleva, E.; Ural Federal University, 19 Mira St., Russian Federation; email: e.g.kovaleva@urfu.ru
Editors	Rieznichenko L.Godymchuk A.
Publisher	American Institute of Physics Inc.
Conference name	13th International Conference of Students and Young Scientists on Prospects of Fundamental Sciences Development, PFSD 2016
Conference date	26 April 2016 through 29 April 2016
Conference code	124417
ISBN	9780735414303
Language of Original Document	English
Abbreviated Source Title	AIP Conf. Proc.
Source	Scopus