In vivo toxicity of copper oxide, lead oxide and zinc oxide nanoparticles acting in different combinations and its attenuation with a complex of innocuous bio-protectors / Minigalieva Ilzira A.,Katsnelson Boris A.,Panov Vladimir G.,Privalova Larisa I.,Varaksin Anatoly N.,Gurvich Vladimir B.,Sutunkova Marina P.,Shur Vladimir Ya.,Shishkina Ekaterina V.,Valamina Irene E.,Zubarev Ilya V.,Makeyev Oleg H.,Meshtcheryakova Ekaterina Y.,Klinova Svetlana V. // TOXICOLOGY. - 2017. - V. 380, l. . - P. 72-93

ISSN/EISSN:

0300-483X / нет данных

Type:

Article

Abstract:

Stable suspensions of metal oxide nanoparticles (Me-NPs) obtained by laser ablation of 99.99\% pure copper, zinc or lead under a layer of deionized water were used separately, in three binary combinations and a triple combination in two independent experiments on rats. In one of the experiments the rats were instilled with Me-NPs intratracheally (i.t.) (for performing a broncho-alveolar lavage in 24 h to estimate the cytological and biochemical indices of the response of the lower airways), while in the other, Me-NPs were repeatedly injected intraperitoneally (i.p.) 18 times during 6 weeks (for estimating the accumulation of corresponding metals in the blood and their excretion with urine and feces and for assessing subchronic intoxication by a large number of functional and morphological indices). Mathematical description of the results from both experiments with the help of the Response Surface Methodology has shown that, as well as in the case of any other binary toxic combinations previously investigated by us, the response of the organism to a simultaneous exposure to any two of the Me-NPs under study is characterized by complex interactions between all possible types of combined toxicity (additivity, subadditivity or superadditivity of unidirectional action and different variants of opposite effects) depending on which effect it is estimated for as well as on the levels of the effect and dose. With any third Me-NP species acting in the background, the type of combined toxicity displayed by the other two may change significantly (as in the earlier described case of a triple combination of soluble metal salts). It is shown that various harmful effects produced by CuO-NP + ZnO-NP + PbO-NP combination may be substantially attenuated by giving rats per os a complex of innocuous bioactive substances theoretically expected to provide a protective integral and/or metal-specific effect during one month before i.t. instillation or during the entire period of i.p. injections. (C) 2017 Elsevier B.V. All rights reserved.

Author keywords:

Nanotoxicology; Combined toxicity; Copper oxide; Zinc oxide; Lead oxide; Bioprotectors INTRATRACHEAL INSTILLATION; METALLIC NANOPARTICLES; SILVER NANOPARTICLES; SUBCHRONIC TOXICITY; COMBINED IMPACTS; CHROMIUM VI; NICKEL; RATS; BIOPROTECTORS; GENOTOXICITY

DOI:

10.1016/j.tox.2017.02.007

Web of Science ID:

ISI:000396947300008

Соавторы в МНС:

Другие поля

Поле	Значение
Month	APR 1
Publisher	ELSEVIER IRELAND LTD
Address	ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
Language	English
Keywords-Plus	INTRATRACHEAL INSTILLATION; METALLIC NANOPARTICLES; SILVER NANOPARTICLES; SUBCHRONIC TOXICITY; COMBINED IMPACTS; CHROMIUM VI; NICKEL; RATS; BIOPROTECTORS; GENOTOXICITY
Research-Areas	Pharmacology \& Pharmacy; Toxicology
Web-of-Science-Categories	Pharmacology \& Pharmacy; Toxicology
Author-Email	bkaznelson@etel.ru
ResearcherID-Numbers	Makeyev, Oleg/G-5511-2017 Shishkina, Ekaterina/I-1829-2017 Shur, Vladimir/J-9078-2015
ORCID-Numbers	Shishkina, Ekaterina/0000-0002-2574-7472
Number-of-Cited-References	67
Usage-Count-Last-180-days	10
Usage-Count-Since-2013	10
Journal-ISO	Toxicology
Doc-Delivery-Number	EO8NX