Studying of cell culture reaction to temperature change by dynamic speckle interferometry / Mikhailova Y.A., Vladimirov A.P., Bakharev A.A., Sergeev A.G., Novosyolova I.A., Yakin D.I. // Russian Journal of Biomechanics. - 2017. - V. 21, l. 1. - P. 64-73.

ISSN:

18125123

Type:

Article

Abstract:

At present, researchers effectively use biospeckles to study the processes occurring inside and on the surface of various biological objects: fruits, vegetables, grains and a patient's skin and retina. Previous research resulted in developing a technique and device for determination of the metabolic activity of Vero, L41 and HLE cell lines using dynamic speckle interferometry. The cell activity parametres were the time-average digital value of radiation intensity Ĩ in a TV camera pixel and the correlation coefficient of two speckle images η. The researchers obtained well-reproducible results in the course of studying herpes virus 1-infected cell lines. In this article, we present an optical technique that allows researchers to determine the standard deviation of the optical wave path difference σu in a small area of an individual cell or in a cell group by using the correlation coefficient of the speckle image fragment. The target of research was a L41 cell culture monolayer on a glass substrate placed in a cuvette with a growth medium. Radiation from a laser module illuminates the cuvette through a mat diffuser; the resulting speckle cell image had an optical magnification of × 11. We used value σu as a parameter characterizing the activity of physical and chemical processes in cells. We showed that there are significant fluctuations of value σu in space and time with temperature T increasing from 26 to 37 °C in an hour. When the temperature stabilizes for 30 min, the fluctuations practically cease, and when the temperature changes by decimal points, there is good correlation between σu and T.

Author keywords:

L41 cell line; Metabolism; Non-destructive testing; Speckle interferometry; Temperature

Index keywords:

нет данных

DOI:

10.15593/RZhBiomeh/2017.1.06

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017406088&doi=10.15593%2fRZhBiomeh%2f2017.1.06&partnerID=40&md5=185bb1aefb04cf622241bf16c4eaf7c8

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Поле	Значение
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017406088&doi=10.15593%2fRZhBiomeh%2f2017.1.06&partnerID=40&md5=185bb1aefb04cf622241bf16c4eaf7c8
Affiliations	Yekaterinburg, Russian Federation
Author Keywords	L41 cell line; Metabolism; Non-destructive testing; Speckle interferometry; Temperature
References	Briers, J.D., Webster, S., Laser speckle contrast analysis (LASCA): a nonscanning, full-field technique for monitoring capillary blood flow (1996) Journal of Biomedical Optics., 1 (2), pp. 174-179; Briers, J.D., Laser speckle contrast imaging for measuring blood flow (2007) Optica Applicata., 37 (1-2), pp. 139-152; Cardoso, R.R., Costa, A.G., Nobre, C.M.B., Braga, R.A., Jr., Frequency signature of water activity by biospeckle laser (2011) Optics Communications., 284 (8), pp. 2131-2136; Choi, W., Fang-Yen, C., Badizadegan, K., Oh, S., Lue, N., Dasari, R.R., Feld, M.S., Tomographic phase microscopy (2007) Nature Methods., 4 (9), pp. 717-719; Fercher, A.F., Briers, J.D., Flow visualization by means of single-exposure speckle photography (1981) Optics Communications., 37 (5), pp. 326-330; Niemz, M.H., Laser-Tissue Interactions: Fundamentals and Applications (1996), Springer; Oulamara, A., Tribillon, G., Duvernoy, J., Biological activity measurement on botanical specimen surfaces using a temporal decorrelation effect of laser speckle (1989) Journal of Modern Optics., 36 (2), pp. 165-179; Rabal, H.J., Braga, R.A., Jr., Dynamic Laser Speckle and Applications (2008), New York: CRC Press
Publisher	Perm National Research Polytechnic University
Language of Original Document	Russian
Abbreviated Source Title	Russ. J. Biomech.
Source	Scopus