Comparative in vivo assessment of some adverse bioeffects of equidimensional gold and silver nanoparticles and the attenuation of nanosilver's effects with a complex of innocuous bioprotectors / Katsnelson B.A., Privalova L.I., Gurvich V.B., Makeyev O.H., Shur V.Y., Beikin Y.B., Sutunkova M.P., Kireyeva E.P., Minigalieva I.A., Loginova N.V., Vasilyeva M.S., Korotkov A.V., Shuman E.A., Vlasova L.A., Shishkina E.V., Tyurnina A.E., Kozin R.V., Valamina I.E., Pichugova S.V., Tulakina L.G. // Interna

ISSN:
16616596
Type:
Article
Abstract:
Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat's pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a "bioprotective complex" (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Author keywords:
Bioprotectors; Bronchoalveolar lavage; Genotoxicity; Nanogold; Nanosilver; Subchronic toxicity
Index keywords:
acetylcysteine; amino acid; calcium; fish oil; glutamate sodium; glycine; gold nanoparticle; multivitamin; pectin; protective agent; selenium; silver nanoparticle; animal cell; animal experiment; anim
DOI:
10.3390/ijms14022449
Смотреть в Scopus:
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875109377&doi=10.3390%2fijms14022449&partnerID=40&md5=cdaa307ceb3e4cfa672683bf81b19eae
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Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875109377&doi=10.3390%2fijms14022449&partnerID=40&md5=cdaa307ceb3e4cfa672683bf81b19eae
Affiliations Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov Str, Ekaterinburg 620014, Russian Federation; Ural State Medical Academy, 17 Klyuchevskaya Str, Ekaterinburg 620109, Russian Federation; Institute of Natural Sciences, Ural Federal University, Ekaterinburg 620000, Russian Federation; City Clinical Diagnostics Centre, 38 Dekabristov Str, Ekaterinburg 620142, Russian Federation
Author Keywords Bioprotectors; Bronchoalveolar lavage; Genotoxicity; Nanogold; Nanosilver; Subchronic toxicity
Chemicals/CAS acetylcysteine, 616-91-1; amino acid, 65072-01-7; calcium, 14092-94-5, 7440-70-2; fish oil, 8016-13-5; glutamate sodium, 142-47-2, 16177-21-2, 16690-92-9; glycine, 56-40-6, 6000-43-7, 6000-44-8; pectin, 9000-69-5; selenium, 7782-49-2
Manufacturers Farmavit, Russian Federation; Promavtomatika, Russian Federation
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Correspondence Address Katsnelson, B. A.; Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov Str, Ekaterinburg 620014, Russian Federation; email: bkaznelson@ymrc.ru
Language of Original Document English
Abbreviated Source Title Int. J. Mol. Sci.
Source Scopus