Uptake of some metallic nanoparticles by, and their impact on pulmonary macrophages in vivo as viewed by optical, atomic force, and transmission electron microscopy / Katsnelson B.A., Privalova L.I., Sutunkova M.P., Khodos M.Y., Shur V.Y., Shishkina E.V., Tulakina L.G., Pichugova S.V., Beikin J.L. // Journal of Nanomedicine and Nanotechnology. - 2012. - V. 3, l. 1.

ISSN:
21577439
Type:
Review
Abstract:
Optical microscopy (OM), semi-contact atomic force microscopy (sc-AFM), and transmission electron microscopy (TEM) were applied to examine cells in the broncho-alveolar lavage fluid (BALF) obtained from rats 24 hours after instillation of different metallic particles suspended in deionised water or of water without any particles. In a comparative experiment with iron oxide Fe3O4 (magnetite) particles having a mean diameter of 10 nm, 50 nm or 1 μm, it was demonstrated that, given equal mass doses, nanoparticles (NPs) induce much more intensive recruitment of phagocytes with a much more significant shift toward neutrophil leukocytes (NL) count in the BALF cell population than micrometric particles do, this shift being an indirect but informative index of particle cytotoxicity for alveolar macrophages (AM). Judging by NL/AM ratio, this cytotoxicity diminishes in the sequence: 10 nm > 50 nm> 1 μm, while judging by OM counts of visible aggregated particles within AMs and NLs and by sc-AFM count of micro-invaginations on the surfaces of these cells, their avidity for particles decreases in the same succession. The same dependence of cell recruitment and of phagocytic activity on NP cytotoxicity was found when the NP diameters were quite similar (ca. 3.5-4.0 nm) but the cytotoxicity of one metal (in our experiment, nanosilver) was higher than that of another (nanogold). TEM pictures of AMs from rats administered the 10 nm magnetite testify to the ability of AMs to actively engulf single NPs and their small aggregates which then form larger conglomerates within fused phagosomes. Some of these large phagosomes lost their membrane, and so freed NPs came into close contact with the nuclear membrane and with mitochondrial membranes and cristae causing their marked damage.& copy 2012 Katsnelson BA, et al.
Author keywords:
Magnetite; Pulmonary phagocytosis; Silver and gold nanoparticles
Index keywords:
нет данных
DOI:
10.4172/2157-7439.1000129
Смотреть в Scopus:
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875104192&doi=10.4172%2f2157-7439.1000129&partnerID=40&md5=aa115cdf9316ee82baaf6eb0ee92cbb2
Соавторы в МНС:
Другие поля
Поле Значение
Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875104192&doi=10.4172%2f2157-7439.1000129&partnerID=40&md5=aa115cdf9316ee82baaf6eb0ee92cbb2
Affiliations Ekaterinburg Medical Research Centre for Prophylaxis and Health Protection in Industrial Workers, Russian Federation; Ural State University of Economics, Russian Federation; Ural Federal University, Russian Federation; Ekaterinburg City Clinical Diagnostics Centre, Russian Federation
Author Keywords Magnetite; Pulmonary phagocytosis; Silver and gold nanoparticles
References Donaldson, K., Stone, V., Tran, C.L., Kreyling, W., Borm, P.J., Nanotoxicology (2004) Occup Environ, 61, pp. 727-728; Oberdörster, G., Oberdörster, E., Oberdörster, J., Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles (2005) Envitonm Health Perspect, 113, pp. 823-839; Bastus, N.G., Casals, E., Vazquez-Compos, S., Puntes, V., Reactivity of Engineered Inorganic Nanoparticles and Carbon Nanostructures in Biological Media (2008) Nanotoxicology, 2, pp. 99-112; Li, N., Xia, T., Nel, A., The Role of Oxidative Stress in Ambient Particulate Matter-Induced Lung Diseases and Its Implications in the Toxicity of Engineered Nanoparticles (2008) Free Radic Bio Med, 44, pp. 1689-1699; Warheit, D.B., Reed, K.L., Sayes, C.M., A role of surface reactivity in TiO2and quartz-related nanoparticle pulmonary toxicity (2009) Nanotoxicology, 3, pp. 181-187; Warheit, D.B., Webb, T.R., Vl, C., Kl, R., Sayes, C.M., Pulmonary bioassay studies with nanoscale and fine-quartz particles in rats: Toxicity is not dependent upon particle size but on surface characteristics (2007) Toxicol Sci, 95, pp. 270-280; Klarisson, H., Gustaffson, J., Cronholm, P., Möller, L., Size-dependent toxicity of metal oxide particles - a comparison between nano- and micrometer size (2009) Toxicol Lett, 188, pp. 112-118; Katsnelson, B., Privalova, L., Kuzmin, S., Degtyareva, T.D., Sutunkova, M.P., Some peculiarities of pulmonary clearance mechanisms in rats after intratracheal instillation of magnetite (Fe3O4) suspensions with different particle sizes in the nanometer and micrometer ranges: Are we defenseless against nanoparticles (2010) Int J Occupat and Environ Health, 16, pp. 508-524; Katsnelson, B.A., Privalova, L.I., Degtyareva, T., Sutunkova, Experimental estimates of the toxicity of iron oxide Fe3O4 (magnetite) nanoparticles (2010) Central Eur J Occup and Envir Medic, 16, pp. 47-63; Katsnelson, B.A., Degtyareva, T.D., Minigalieva, I.I., Privalova, L.I., Kuzmin, S., Sub-chronic systemic toxicity and bio-accumulation of Fe3O4 nano- and microparticles following repeated intraperitoneal administration to rats (2010) Internat J Toxicol, 30, pp. 59-68; Katsnelson, B.A., Privalova, L.I., Sutunkova, M.P., Tulakina, L.G., The "In Vivo" Interaction Between Iron Oxide Fe3O4 Nanoparticles And Alveolar Macrophages (2011) Bull Exptl Biol Medic, 151 (11), pp. 560-565; Katsnelson, B.A., Privalova, L.I., Recruitment of Phagocytizing Cells into the Respiratory Tract as a Response to the Cytotoxic Action of Deposited Particles (1984) Environ Health Perspect, 55, pp. 313-325; Privalova, L.I., Katsnelson, B.A., Yelnichnykh, L.N., (1987) Brit J Ind Med, 44, pp. 228-235. , some peculiarities of the pulmonary phagocytotic response, dust kinetics, and silicosis development during long term exposure of rats to high quartz levels; Privalova, L.I., Katsnelson, B.A., Osipenko, A.B., Yushkov, B.N., Babushkina, L.G., Response of a phagocyte cell system to products of macrophage breakdown as a probable mechanism of alveolar phagocytosis adaptation to deposition of particles of different cytotoxicity (1980) Environm Health Perspect, 35, pp. 205-218; Privalova, L.I., Katsnelson, B.A., Sharapova, N.Y., Kislitsina, N.S., On the relationship between activation and the breakdown of macrophages in pathogenesis of silicosis (1995) Medic Lavoro, 86, pp. 511-521; Katsnelson, B.A., Konyscheva, L.K., Sharapova, N.Y.E., Privalova, L.I., Prediction of the comparative intensity of pneumoconiotic changes caused by chronic inhalation exposure to dusts of different cytotoxicity by means of a mathematical model (1994) Occup Environ Med, 51, pp. 173-180; Katsnelson, B.A., Konysheva, L., Privalova, L.I., Sharapova, N.Y., Quartz dust retention in rat lungs under chronic exposure simulated by a multicompartmental model: Further evidence of the key role of the cytotoxicity of quartz particles (1997) Inhalation Toxicology, 9, pp. 703-715; Katsnelson, B.A., Alekseyeva, O.G., Privalova, L.I., Polzik, E.V., Pneumoconioses: The Pathogenesis And Biological Prophylaxis (1995) Ekaterinburg: The Urals Division of the Ras, p. 325; Ahamed, M., Alsalhi, M.S., Siddiqui, M.K., Silver Nanoparticles Applications and human health (2010) Clinica Chimica Acta, 411 (23-24), pp. 1841-1848; Ahmadi, F., Kordestany, A.H., Investigation On Silver Retention In Different Organs And Oxidative Stress Enzymes In Male Broiler Fed Diet Supplemented With Powder Of Nano Silver (2011) Amer-Eurasian J Toxicol Sci, 3, pp. 28-35; Asharani, P., Lianwu, Y., Gong, Z., Valiyaveettil, S., Comparison Of The Toxicity Of Silver, Gold And Platinum Nanoparticles In Developing Zebra Fish Embryos (2010) Nanotoxicology, 5, pp. 43-54; Bakri, S.J., Pulido, J.S., Mukerjee, P., Marler, R.J., Mukhopadhyay, D., Absence of histologic retinal toxicity of intravitreal nanogold in a rabbit model (2008) Retina, 28, pp. 147-149; Balasurbamanian, S., Jittiwat, J., Manikandan, J., Ong, C.N., Yu, L.E., Biodistribution of gold nanoparticles and gene expression changes in the liver and spleen after intravenous administration in rats (2010) Biomater, 31, pp. 2034-2042; Chen, Y.S., Hung, Y.C., Liau, I., Huang, G.S., Assessment Of The In Vivo Toxicity Of Gold Nanoparticles (2009) Nanoscale Res Lett, 8, pp. 858-864; Glazer, E.S., Zhu, C., Hamir, A., Borne, A., Thompson, C.S., Curley, S.A., Biodistribution And Acute Toxicity Of Naked Gold Nanoparticles In A Rabbit Hepatic Tumor Model (2010) Nanotoxicology, 5, pp. 459-468; Park, E.J., Bae, E., Yi, J., Kim, Y., Choi, K., Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nano-particles (2010) Environ Toxicol Pharmacol, 30, pp. 162-168; Mustafa, T., Watanabe, F., Monroe, W., Impact Of Gold Nanoparticle Concentration On Their Cellular Uptake By Mc3t3-E1 Mouse Osteoblastic Cells As Analyzed By Transmission Electron Microscopy (2011) J Nanomedic Nanotechnol, 2, p. 118; Lurje, Y., (1973) Standardized Methods For Analyzing Waters, , Moscow: Publishing House "Khimiya" (Russian); Allison, A.C., Lysosomes And The Toxicity Of Particulate Pollutants (1971) Arch Intern Med, 128, pp. 131-139
Correspondence Address Katsnelson, B. A.; Ekaterinburg Medical Research Centre for Prophylaxis and Health Protection in Industrial WorkersRussian Federation; email: bkaznelson@etel.ru
Language of Original Document English
Abbreviated Source Title J. Nanomedicine Nanotechnology
Source Scopus