Mossbauer and positron life-time study of oxyhemoglobin solution irradiated by electrons / Oshtrakh M.I., Semionkin V.A., Kopelyan E.A., Milder O.B. // Radiation Physics and Chemistry. - 1999. - V. 55, l. 5-6. - P. 549-554.

ISSN:
0969806X
Type:
Conference Paper
Abstract:
Human oxyhemoglobin concentrated solution exposed to electrons with average energy of 10 MeV in doses of 1.7, 2.5 and 5.0 kGy was studied by Mossbauer spectroscopy and positron life-time technique. Precise measurements of Mossbauer spectra of irradiated oxyhemoglobin samples revealed deoxygenation process and formation of two ferric compounds at 1.7 kGy and ferrous and ferric compounds at 2.5 and 5.0 kGy. Positron life-time parameters showed decrease of the free volume radius and the total free volume in irradiated oxyhemoglobin solutions.Human oxyhemoglobin concentrated solution exposed to electrons with average energy of 10 MeV in doses of 1.7, 2.5 and 5.0 kGy was studied by Mossbauer spectroscopy and positron life-time technique. Precise measurements of Mossbauer spectra of irradiated oxyhemoglobin samples revealed deoxygenation process and formation of two ferric compounds at 1.7 kGy and ferrous and ferric compounds at 2.5 and 5.0 kGy. Positron life-time parameters showed decrease of the free volume radius and the total free volume in irradiated oxyhemoglobin solutions.
Author keywords:
Index keywords:
Atomic spectroscopy; Dosimetry; Electron irradiation; Iron compounds; Mossbauer spectroscopy; Positrons; Radiation damage; Free volume; Oxyhemoglobin; Positron lifetime technique; Hemoglobin; deoxyhem
DOI:
10.1016/S0969-806X(99)00300-X
Смотреть в Scopus:
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033178751&doi=10.1016%2fS0969-806X%2899%2900300-X&partnerID=40&md5=51b1b69d650b5a2aa2b3b155ead26b13
Соавторы в МНС:
Другие поля
Поле Значение
Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033178751&doi=10.1016%2fS0969-806X%2899%2900300-X&partnerID=40&md5=51b1b69d650b5a2aa2b3b155ead26b13
Affiliations Division of Applied Biophysics, Fac. Phys. Techniques Devices Q., Sverdlovsk, Russian Federation; Faculty of Experimental Physics, Ural State Tech. University, 620002, Sverdlovsk, Russian Federation
Chemicals/CAS ferric ion, 20074-52-6; ferrous ion, 15438-31-0; oxyhemoglobin, 9061-63-6
References Ashry, H.A., Selim, N.S., El-Behay, A.Z., The effect of gamma-rays on the hemoglobin of whole-body irradiated mice (1994) Radiat. Phys. Chem, 44, p. 187; Chevalier, A., Kellershohn, C., Rimbert, J.N., X-irradiation effect on human red cells: Hemoglobin study by Mössbauer spectrometry (1983) Radiat. Res, 94, p. 51; El Din, S., Aisha, A., El Behay, A.Z., Effect of gamma irradiation on infrared spectra of rat hemoglobin (1994) Radiat. Phys. Chem, 44, p. 195; El Din, A.A.S., Desouky, O.S., El Behay, A.Z., El Sayed, A.A., Biophysical study of mice after whole body irradiation (1996) Radiat. Phys. Chem, 47, p. 755; Eldrup, M., Lightboody, D., Sherwood, J.N., Studies of phase transformations in molecular crystals using the positron annihilation technique (1981) Chem. Phys, 63, p. 51; Irkaev, S.M., Kupriyanov, V.V., Semionkin, V.A., (1987), Method of registration of nuclear γ-resonance. British Patent No 10745; Kellershohn, C., Rimbert, J.N., Chevalier, A., Hubert, C., Comparative study of oxyhemoglobin radiolysis and thermolysis by Mössbauer spectroscopy (1976) J. de Phys, 37, pp. 6-185; Kozlov, A.A., Berkovsky, A.L., Smirnova, I.L., Gorbunova, N.A., Balakina, T.A., (1985), Method of hemoglobin preparation. Patent SU 1205910 A; Myshkin, A.E., Sharpatiy, V.A., Role of structural factor at radiolytic damage of human hemoglobin (1996) Biophysika (Moscow), 31, p. 592; Oshtrakh, M.I., Semionkin, V.A., Mössbauer effect study of gamma-irradiated human oxyhemoglobin (1991) Radiat. Environ. Biophys, 30, p. 33; Oshtrakh, M.I., Semionkin, V.A., Oxyhemoglobin radiolysis studied by Mössbauer spectroscopy (1992) Nuclear Medicine. Nuclear Medicine in Research and Practice, pp. 285-288. , H.A.E. Schmidt, & R. Höfer. Stuttgart: Schattauer; Oshtrakh, M.I., Kopelyan, E.A., Semionkin, V.A., Oxyhemoglobin degradation and radiolysis analysed by Mössbauer and positron annihilation techniques (1993) Anal. Chim. Acta, 279, p. 179; Oshtrakh, M.I., Kopelyan, E.A., Semionkin, V.A., Mössbauer and positron annihilation studies of structural modifications of hemoglobin in solution (1995) J. Radioanal. Nucl. Chem., Articles, 190, p. 243; Oshtrakh, M.I., Kopelyan, E.A., Semionkin, V.A., Mössbauer and positron annihilation study of oxyhemoglobin exposed to γ-rays and electrons (1996) Radiat. Phys. Chem, 47, p. 399; Oshtrakh, M.I., Kopelyan, E.A., Semionkin, V.A., Mössbauer and positron life-time spectroscopy of γ- And electron-irradiated oxyhemoglobin solution (1997) Nucl. Instrum. Meth. Phys. Res, 131, p. 226; Oshtrakh, M.I., Semionkin, V.A., Khleskov, V.I., Galtsev, M.V., Study of gamma-irradiation effect on oxyhemoglobin by Mössbauer spectroscopy (1991) Questions of the Application of Nuclear Radiations, pp. 140-146. , YuF Babikova. Moscow: Energoatomizdat. (in Russian); Suchomlinov, B.F., Savich, A.V., Starikovich, L.S., Dudok, E.P., Radiation induced structural and functional modification of hemoglobin (1985) Gen. Physiol. Biophys, 4, p. 301; Szweda-Lewandowska, Z., Puchala, M., Leyko, W., Effects of gamma irradiation on the structure and function of human hemoglobin (1976) Radiat. Res, 65, p. 50; Szweda-Lewandowska, Z., Puchala, M., Osmulski, P.A., Radiation-induced changes of structural and functional properties of human hemoglobin. I. Spectral characterization of irradiated deoxyhemoglobin (1989) Radiat. Environ. Biophys, 28, p. 39; Szweda-Lewandowska, Z., Puchala, M., Osmulski, P.A., Rosin, J., Radiation-induced changes of structural and functional properties of human hemoglobin. II. Structural and functional characterization of irradiated deoxyhemoglobin (1989) Radiat. Environ. Biophys, 28, p. 47
Correspondence Address Oshtrakh, M.I.; Division of Applied Biophysics, Fac. Phys. Tech./Devices Qual. Ctrl., Ural State Technical University, Sverdlovsk 620002, Russian Federation; email: oshtrakh@soek.erl.e-burg.su
Publisher Elsevier Science Ltd, Exeter, United Kingdom
Conference name Proceedings of the 1998 9th Tihany Symposium on Radiation Chemistry
Conference date 29 August 1998 through 3 September 1998
Conference location Tata, Hung
Conference code 55530
CODEN RPCHD
Language of Original Document English
Abbreviated Source Title Radiat. Phys. Chem.
Source Scopus