Mössbauer effect study of gamma-irradiated human oxyhemoglobin / Oshtrakh M.I., Semionkin V.A. // Radiation and Environmental Biophysics. - 1991. - V. 30, l. 1. - P. 33-44.

ISSN:
0301634X
Type:
Article
Abstract:
Preliminary results of the Mössbauer effect study of human adult oxyhemoglobin in erythrocytes exposed to gamma-irradiation with doses of ∼ 100, ∼ 300 and ∼ 600 kGy are presented. Mössbauer spectra measured at 87 K have been analyzed in two ways. At first, to fit these spectra we used the four components oxyhemoglobin, deoxyhemoglobin, hemochromes and nonheme Fe(III) compound which had been obtained earlier from Mössbauer spectra of X-irradiated oxyhemoglobin by Chevalier et al. (1983). However, this approximation was not satisfactory. Then a new model of spectral fitting with five components was used. These were oxyhemoglobin, deoxyhemoglobin and components marked 1, 2 and 3. Using Mössbauer hyperfine parameters of each component the valence/spin states of iron ions were determined and possible complexes were considered. The most probable compounds for components 1, 2 and 3 were hematin and/or μ-oxodimers, methemoglobin hydroxide and/or hemichromes, and the high spin Fe(III) complex, respectively. Changes of the relative areas of Mössbauer subspectra of all components (its content in samples) versus doses were evaluated and the presence of the high and low spin aquomethemoglobin was indicated. © 1991 Springer-Verlag.
Author keywords:
Index keywords:
oxyhemoglobin; hemoglobin; article; gamma radiation; human; human tissue; mossbauer spectroscopy; adult; erythrocyte; gamma radiation; metabolism; methodology; Mossbauer spectroscopy; radiation exposu
DOI:
10.1007/BF01595572
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Affiliations Division of Applied Biophysics, Sverdlovsk, 620002, Russia; Faculty of Experimental Physics, Ural Polytechnical Institute, Sverdlovsk, 620002, Russia
Chemicals/CAS hemoglobin, 9008-02-0; oxyhemoglobin, 9061-63-6; Hemoglobins; Oxyhemoglobins
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Correspondence Address Oshtrakh, M.I.; Division of Applied Biophysics, Sverdlovsk, 620002, Russia
Publisher Springer-Verlag
CODEN REBPA
PubMed ID 2000444
Language of Original Document English
Abbreviated Source Title Radiat Environ Biophys
Source Scopus