Retrofit Device for Real-Time Evaluation of Metabolic Activity in Herpes Virus Infected Cell Cultures / Vladimirov A.P., Malygin A.S., Mikhailova Y.A., Bakharev A.A., Poryvaeva A.P. // Biomedical Engineering. - 2014. - V. 48, l. 4. - P. 178-181.

ISSN:

00063398

Type:

Article

Abstract:

A speckle interferometry device for evaluation of the metabolic activity of cells cultivated on a transparent substrate was upgraded, fine-tuned, and tested. The cell cultures L-41 CD/84, Vero, and LEH-3 infected with herpes simplex virus (HSV) and placed in a cultivation medium were studied. The upgrading included introduction of time averaging of the signals and real time signal processing. It was demonstrated that the degree of correlation of fragments of the speckle image taken at two moments in time could be used as a parameter characterizing the metabolic activity of the cells. © 2014, Springer Science+Business Media New York.

Author keywords:

Index keywords:

Cells; Metabolism; Signal processing; Speckle; Viruses; Degree of correlations; Herpes simplex virus; Metabolic activity; Parameter characterizing; Real-time signal processing; Speckle interferometry;

DOI:

10.1007/s10527-014-9447-9

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925491940&doi=10.1007%2fs10527-014-9447-9&partnerID=40&md5=701250b4bc5babe6a9f09593b1cbcf95

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Другие поля

Поле	Значение
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925491940&doi=10.1007%2fs10527-014-9447-9&partnerID=40&md5=701250b4bc5babe6a9f09593b1cbcf95
Affiliations	Physical and Technological Institute, Urals Federal University, Yekaterinburg, Russian Federation; Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Yekaterinburg, Russian Federation
References	Rabal, H.J., (2008) Dynamic Laser Speckle and Applications, , Braga (eds.) CRC Press; Cardoso, R.R., Costa, A.G., Nobre, C.M.B., Braga, R.A., Jr., (2011) Optics Commun., 284, pp. 2131-2136; Vladimirov, A.P., Lisin, A.L., Mikushin, V.I., (2000) Pis’ma ZhTF, 26, pp. 20-24; Fercher, A.F., Briers, J.D., (1981) Optics Commun., 37, pp. 326-330; Briers, J.D., Webster, S., (1996) J. Biomed. Optics, 1, pp. 174-179; Briers, J.D., (2007) Optica Applicata, 37, pp. 139-152; Vladimirov, A.P., Druzhinin, A.V., Malygin, A.S., Mikitas, K.N., (2012) Proc. SPIE., 8337, pp. 1-15; Malygin, A.S., Bebenina, N.V., Vladimirov, A.P., Mikitas, K.N., Bakharev, A.A., (2012) PTE, 3, pp. 124-127; (1999) Catalog of Russian Collection of Cellular Cultures, , [in Russian], OmGPU, Omsk; RF Patent No. 2148644; Bocharov, A.F., Kitsak, V.Y., Bocharov, E.F., Trukhachev, A.A., (1992) Herpes Simplex Virus [in Russian], , Nauka, Novosibirsk:
Correspondence Address	Vladimirov, A.P.; Physical and Technological Institute, Urals Federal UniversityRussian Federation
Publisher	Springer New York LLC
CODEN	BIOEA
Language of Original Document	English
Abbreviated Source Title	Biomed. Eng.
Source	Scopus