Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells / Vladimirov A.P., Malygin A.S., Mikhailova J.A., Borodin E.M., Bakharev A.A., Poryvayeva A.P. // Journal of Physics: Conference Series. - 2014. - V. 536, l. 1.

ISSN:

17426588

Type:

Conference Paper

Abstract:

Earlier we reported developing a speckle interferometry technique and a device designed to assess the metabolic activity of a cell monolayer cultivated on a glass substrate. This paper aimed at upgrading the technique and studying its potential for real-time assessment of herpes virus development process. Speckle dynamics was recorded in the image plane of intact and virus-infected cell monolayer. HLE-3, L-41 and Vero cells were chosen as research targets. Herpes simplex virus-1-(HSV-1)- infected cell cultures were studied. For 24 h we recorded the digital value of optical signal I in one pixel and parameter η characterizing change in the distribution of the optical signal on 10 × 10-pixel areas. The coefficient of multiple determination calculated by η time dependences for three intact cell cultures equals 0.94. It was demonstrated that the activity parameters are significantly different for intact and virus-infected cells. The difference of η value for intact and HSV-1-infected cells is detectable 10 minutes from the experiment start. © Published under licence by IOP Publishing Ltd.

Author keywords:

Index keywords:

Cell culture; Image processing; Interferometry; Metabolism; Monolayers; Optical data processing; Optical signal processing; Speckle; Substrates; Viruses; Development process; Glass substrates; Herpes

DOI:

10.1088/1742-6596/536/1/012012

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907259496&doi=10.1088%2f1742-6596%2f536%2f1%2f012012&partnerID=40&md5=b5e44db1a5e6f6efdcad7af8968671f4

Соавторы в МНС:

Другие поля

Поле	Значение
Art. No.	012012
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907259496&doi=10.1088%2f1742-6596%2f536%2f1%2f012012&partnerID=40&md5=b5e44db1a5e6f6efdcad7af8968671f4
Affiliations	Ural Federal University, Mira street 19, Yekaterinburg, Russian Federation; FSSI Yekaterinburg Research Institute of Viral Infections of Rospotrebnadzor, Letnaya Street 23, Yekaterinburg, Russian Federation
References	(2008) Dýnamic Laser Speckle and Applications; Cardoso, R.R., Costa, A.G., Nobre, C.M.B., Braga, R.A., Frequency signature of water activity by biospeckle laser (2011) Opt. Commun., 284 (8), pp. 2131-2136. , 0030-4018; Oulamara, Tribillon, G., Duvernoy, J., Biological activity measurement on botanical specimen surfaces using a temporal decorrelation effect of laser speckle (1989) J. Mod. Opt., 36 (2), pp. 165-179. , 0950-0340; Briers, J.D., Laser speckle contrast imaging for measuring blood flow (2007) Optica Applicata, 37, pp. 139-152. , 0078-5466; Malygin, A.S., Bebenina, N.V., Vladimirov, A.P., Mikitas, K.N., Baharev, A.A., A speckle- interferometric device for studying the cell biological activity (2012) Instrum. Experimental Techniques, 55 (3), pp. 415-418. , 0020-4412
Correspondence Address	Vladimirov, A.P.; Ural Federal University, Mira street 19, Russian Federation
Editors	Gurov I.P.Kozlov S.A.
Publisher	Institute of Physics Publishing
Conference name	1st International Scientific School on Methods of Digital Image Processing in Optics and Photonics
Conference date	8 April 2014 through 11 April 2014
Conference code	114528
Language of Original Document	English
Abbreviated Source Title	J. Phys. Conf. Ser.
Source	Scopus