A speckle-interferometric device for studying the cell biological activity / Malygin A.S., Bebenina N.V., Vladimirov A.P., Mikitas K.N., Baharev A.A. // Instruments and Experimental Techniques. - 2012. - V. 55, l. 3. - P. 415-418.

ISSN:

00204412

Type:

Article

Abstract:

A modernized device intended for diagnosing the temperature-dependent activity of cells is described. The activity of cells was determined by finding the correlation coefficient of optical signals in speckle patterns recorded at different moments of time. It is shown that, as the temperature increases from 26 to 36Y°C, variations in the optical paths in cells increase from 16 to 26 nm. This is probably associated with the physiological activity of cells-trapping of large molecules or their conglomerates. In contrast to the pre- vious variant of the device design, instead of a lipid membrane, a cell monolayer was used, which was culti- vated on a plane-parallel glass plate. In order to reduce variations in the optical path lengths in a nutrient solution, a 1.7-mm-thick plane-parallel glass plate was additionally inserted into the cuvette. The modern- ization of the device also includes heating of the cuvette with hot air flows. © Pleiades Publishing, Ltd., 2012.

Author keywords:

Index keywords:

Cell monolayers; Correlation coefficient; Device design; Glass plate; Hot airflow; Lipid membranes; Nutrient solution; Optical path; Optical path lengths; Optical signals; Physiological activity; Redu

DOI:

10.1134/S0020441212020091

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865576865&doi=10.1134%2fS0020441212020091&partnerID=40&md5=f30e62626d5a4757eca1b71617e522a5

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Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84865576865&doi=10.1134%2fS0020441212020091&partnerID=40&md5=f30e62626d5a4757eca1b71617e522a5
Affiliations	Institute of Physical Engineering, Ural Federal University, ul. Mira 19, Yekaterinburg, Russian Federation; Federal Budget Scientific Institution, Yekaterinburg Research Institute of Virus Infections, Federal Service on Customer's Rights Protection and HumanWellbeing Surveillance, ul. Letnyaya 23, Yekaterinburg, Russian Federation
References	Da Silva, M.M., De Almeida Nozela, J.R., Chaves, M.J., (2011) Opt. Commun., 284, pp. 1798-1802; Cardoso, R.R., Costa, A.G., Batista Nobre, C.M., Alves Braga Jr., R., (2011) Opt. Commun., 284, pp. 2131-2136; Martí-López, L., Cabrera, H., Martínez-Celorio, R.A., González-Peña, R., (2010) Opt. Commun., 283 (24), p. 4972; Ivkov, V.G., Berestovskii, G.N., (1978) Dinamicheskaya Struktura Lipidnogo Bisloya, , Dynamical Structure of Lipid Bilayer Moscow: Nauka; Antonov, V.F., Smirnova, E.Yu., Shevchenko, E.V., (1992) Lipidnye Membrany Pri Fazovykh Prevrashcheniyakh, , Lipid Membranes during Phase Transformations Moscow: Nauka; Bebenina, N.V., Mikitas, K.N., Malygin, A.S., (2011) Problemy Spektroskopii i Spektrometrii: Mezhvuzovskii Sbornik Nauchnykh Trudov, (28), p. 192. , Coll. Sci. Papers "Problems of Spectroscopy and Spectrometry Yekaterinburg: UrFU; Vladimirov, A.P., (2006) 10i Mezhdunarodnyi Seminar- Yarmarka Rossiiskie Tekhnologii Dlya Industrii;, , Sbornik proektov Coll. Projects "Russ. Technol. for Industry," Proc. 10th Int. Semin. Market), St. Petersburg: Tsentr Podderzhki Innovatsii; Vladimirov, A.P., Malygin, A.S., Pavlov, T.N., (2007) 11i Mezhdunarodnyi Seminar-Yarmarka Rossiiskie Tekhnologii Dlya Industrii Sbornik proektov Coll. Projects "Russ. Technol. for Industry," Proc. 11th Int. Semin. Market, , St. Petersburg: Tsentr Podderzhki Innovatsii; Glinskikh, N.P., Vissarionov, V.A., Baharev, A.A., (2000) RF Patent no. 2148644; Adams, R.G., (1980) Cell Cultures for Biochemists, 1983. , Amsterdam: Elsevier Moscow: Mir; Vladimirov, A.P., (2004) Dinamicheskaya Spekl-Interferometriya Deformiruemykh Tel, , Dynamic Speckle-Interferometry of Deformed Bodies, Yekaterinburg: Ural. Otd. Ross. Akad. Nauk
Correspondence Address	Malygin, A.S.; Institute of Physical Engineering, Ural Federal University, ul. Mira 19, Yekaterinburg, Russian Federation; email: somt@bk.ru
Language of Original Document	English
Abbreviated Source Title	Instrum. Exp. Tech.
Source	Scopus