Treatment of archive stored autopsy material for electron microscopy (Russian) / Gaidamakin N.A., Soloviev V.I., Borisova N.P. // Arkhiv Patologii. - 1974. - V. 36, l. 10. - P. 77-80.

ISSN:

00041955

Type:

нет данных

Abstract:

Electron microscopic studies of tissue fixed in formalin and stored for a long time, enabled some peculiar forms of pathologic tissue, such as extremely rare neoplasms, to be investigated. Tissues were washed for 3 to 4 hr in a 4.5% sugar solution prepared in a phosphate buffer pH 7.4; then they were fixed for 12 to 24 hr in an 0.5% solution of OsO4 prepared in this sugar solution with the addition of K2Cr2O7 up to a concentration of 0.5%. In the course of the treatment the sections were contrasted in a piece of uranil acetate added to the third portion of 70° alcohol up to a concentration of 1%; epon or araldite were poured over and the sections were once more contrasted on a screen for a maximum of 10 min with a warm solution (25°C) of lead citrate.

Author keywords:

Index keywords:

formaldehyde; autopsy; electron microscopy; histology; tissue fixation; tissue preservation; article; cytology; glomus tumor; human; methodology; muscle tumor; pathology; staining; stomach tumor; Auto

DOI:

нет данных

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0016199393&partnerID=40&md5=16d7b0e12c3dcb165fc93bbb4b6db43c

Соавторы в МНС:

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Другие поля

Поле	Значение
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0016199393&partnerID=40&md5=16d7b0e12c3dcb165fc93bbb4b6db43c
Chemicals/CAS	formaldehyde, 50-00-0
CODEN	ARPTA
PubMed ID	4142382
Language of Original Document	Russian
Abbreviated Source Title	ARKH. PATOL.
Source	Scopus