Effect of a New Class of Compounds of the Group of Substituted 5R1, 6H2-1,3,4-thiadiazine-2-amines on the Inflammatory and Cytokine Response in Experimental Myocardial Infarction / Sarapultsev Alexey P.,Chupakhin Oleg N.,Sarapultsev Petr A.,Rantsev Maxim A.,Medvedeva Svetlana U.,Sidorova Larisa P. // CURRENT VASCULAR PHARMACOLOGY. - 2015. - V. 13, l. 1. - P. 43-53.

ISSN/EISSN:

1570-1611 / 1875-6212

Type:

Article

Abstract:

This study investigated the effects of the L-17 compound of the group of substituted 5R1, 6H2-1,3,4-thiadiazine-2-amines on the immune response and the plasma level of circulating cytokines in acute myocardial infarction (MI) in rats. The study was based upon experimental work which demonstrated the role of local and systemic inflammatory reactions in MI. Acute MI in rats was induced by left coronary artery coagulation. Histological study of the myocardium sections has been carried out at the 1st and 7th days of the experimental myocardial infarction. Serum activity of creatine phosphokinase (CPK), aspartate aminotransferase (AST), isoenzymes 1 and 2 and lactate dehydroge nase (LDH1-2) were investigated at days 1 and 7. ELISA analysis for plasma cytokine levels was performed using commercially available test kits following the manufacturer's instructions. Biochemical analysis in animals with the administration of the L-17 compound after MI showed that the AST and CPK levels at days 5 and 7 of experiments did not differ significantly from the values of intact animals. In animals of the group with MI without the administration of the L-17 compound, the IL-1 level 8 times and the TNF level 7.8 times exceeded the normal indicators, while the use of L-17 compound in the therapy resulted in only 1.8 times increase of IL-1 level and 4.7 times increase of TNF level in comparison with the norm. Thus, the introduction of L-17 compound in case of experimental MI delays exudative/alternative phase of inflammation, accelerates granulocytic and decreased the inflammation and anti-inflammation interleukins level.

Author keywords:

Cytokines; inflammation; L-17 compound; myocardial infarction TUMOR-NECROSIS-FACTOR; CHRONIC HEART-FAILURE; FACTOR-ALPHA; SOLUBLE RECEPTOR; LEUKOCYTE RECRUITMENT; TRANSGENIC MICE; INTERLEUKIN-10; IL-6; ACTIVATION; EXPRESSION

DOI:

нет данных

Web of Science ID:

ISI:000350754600007

Соавторы в МНС:

Другие поля

Поле	Значение
Publisher	BENTHAM SCIENCE PUBL LTD
Address	EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES
Language	English
EISSN	1875-6212
Keywords-Plus	TUMOR-NECROSIS-FACTOR; CHRONIC HEART-FAILURE; FACTOR-ALPHA; SOLUBLE RECEPTOR; LEUKOCYTE RECRUITMENT; TRANSGENIC MICE; INTERLEUKIN-10; IL-6; ACTIVATION; EXPRESSION
Research-Areas	Pharmacology \& Pharmacy; Cardiovascular System \& Cardiology
Web-of-Science-Categories	Pharmacology \& Pharmacy; Peripheral Vascular Disease
Author-Email	a.sarapultsev@iip.uran.ru
ResearcherID-Numbers	Sarapultsev, Alexey/K-7220-2012
ORCID-Numbers	Sarapultsev, Alexey/0000-0003-3101-9655
Funding-Acknowledgement	Special Research Grant of Interdisciplinary project of the Institute of Immunology and Physiology; Institute of Organic Synthesis, Ural Branch of RAS {[}09-M-34-2002]
Funding-Text	This study was supported by a Special Research Grant of Interdisciplinary project of the Institute of Immunology and Physiology and the Institute of Organic Synthesis, Ural Branch of RAS (grant \# 09-M-34-2002).
Number-of-Cited-References	55
Usage-Count-Last-180-days	2
Usage-Count-Since-2013	10
Journal-ISO	Current Vascular Pharmacology
Doc-Delivery-Number	CD0IN