An enzyme-free electrochemical method for the determination of E-coli using Fe3O4 nanocomposites with a SiO2 shell modified by ferrocene / Kozitsina A. N.,Malysheva N. N.,Utepova I. A.,Glazyrina Yu. A.,Matern A. I.,Brainina Kh. Z.,Chupakhin O. N. // JOURNAL OF ANALYTICAL CHEMISTRY. - 2015. - V. 70, l. 5. - P. 540-545.

ISSN/EISSN:

1061-9348 / 1608-3199

Type:

Article

Abstract:

An electrochemical immunoassay method is developed for determining the strain of Escherichia coli ATCC 25922 with the use of electroactive nanocomposite particles based on magnetite Fe3O4 coated by SiO2 modified with ferrocene as a signal label. A sensitive, direct, and easily measured analytical response is obtained by cyclic voltammetry. The developed method enables the determination of bacterial cells at a minimum concentration of 2.3 x 10(3) CFU/mL. The analytical range is 2.3 x 10(3)-2.3 x 10(7) CFU/mL; the detection limit is 2.5 x 10(2) CFU/mL. The required sample volume is 0.5 mL, and the analysis time is 40 min.

Author keywords:

cyclic voltammetry; immunoassay; electroactive nanocomposite particles QUARTZ-CRYSTAL MICROBALANCE; SENSITIVE DETECTION; MASS-SPECTROMETRY; REAL-TIME; RAPID DETECTION; SALMONELLA; NANOPARTICLES; IMMUNOASSAY; PATHOGEN; SAMPLES

DOI:

10.1134/S1061934815050068

Web of Science ID:

ISI:000353288100003

Соавторы в МНС:

Другие поля

Поле	Значение
Month	MAY
Publisher	MAIK NAUKA/INTERPERIODICA/SPRINGER
Address	233 SPRING ST, NEW YORK, NY 10013-1578 USA
Language	English
EISSN	1608-3199
Keywords-Plus	QUARTZ-CRYSTAL MICROBALANCE; SENSITIVE DETECTION; MASS-SPECTROMETRY; REAL-TIME; RAPID DETECTION; SALMONELLA; NANOPARTICLES; IMMUNOASSAY; PATHOGEN; SAMPLES
Research-Areas	Chemistry
Web-of-Science-Categories	Chemistry, Analytical
Author-Email	a.n.kozitsina@ustu.ru
ResearcherID-Numbers	Kozitsina, Alisa/S-6317-2017 Malysheva, Natalia/A-3667-2015 Glazyrina, Yulia/M-8857-2016
ORCID-Numbers	Malysheva, Natalia/0000-0002-3606-9216
Number-of-Cited-References	37
Usage-Count-Last-180-days	6
Usage-Count-Since-2013	32
Journal-ISO	J. Anal. Chem.
Doc-Delivery-Number	CG4WL