Hybrid Electrochemical/Magnetic Assay for Salmonella Typhimurium Detection / Brainina Khiena Z.,Kozitsina Alisa N.,Glazyrina Yulia A. // IEEE SENSORS JOURNAL. - 2010. - V. 10, l. 11. - P. 1699-1704.

ISSN/EISSN:

1530-437X / нет данных

Type:

Article

Abstract:

Electrochemical immunoassay involving two magnetic steps-separation of unbound nanoparticles from bacteria/antigen-Fe(3)O(4) nanoparticles conjugates and concentrating of the conjugates on solid substrate is proposed. Carbon screen printed electrodes covered antibodies specific to Salmonella typhimurium (strain SL 7207) serves as the mentioned substrate. Immunocomplex formed is being solved in the mixture of acids (0.36 M H(2)SO(4) and 0.28 M HNO(3)) is analyzed for Fe(3+) concentration. The last is determined by stripping voltammetric method. It provides information on quantity of conjugates localized on the substrate and the last one is proportional to the pathogen concentration in the analyte. Detection limit is for Salmonella typhimurium 8.18 CFU/ml and for antigen Salmonella typhimurium 1.51 x 10(-7) mg/ml (1.51 CFU/ml).

Author keywords:

Immunosensor; magnetic nanoparticles; Salmonella typhimurium; voltammetry ESCHERICHIA-COLI O157; IMMUNOSENSOR; LABEL; GOLD; NANOPARTICLES; PROTEINS; FILMS

DOI:

10.1109/JSEN.2010.2046410

Web of Science ID:

ISI:000283291000004

Соавторы в МНС:

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Другие поля

Поле	Значение
Month	NOV
Publisher	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Address	445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Language	English
Keywords-Plus	ESCHERICHIA-COLI O157; IMMUNOSENSOR; LABEL; GOLD; NANOPARTICLES; PROTEINS; FILMS
Research-Areas	Engineering; Instruments \& Instrumentation; Physics
Web-of-Science-Categories	Engineering, Electrical \& Electronic; Instruments \& Instrumentation; Physics, Applied
Author-Email	baz@usue.ru kozitsina@mail.ustu.ru glazirina@usue.ru
ResearcherID-Numbers	Glazyrina, Yulia/M-8857-2016 Kozitsina, Alisa/S-6317-2017
Funding-Acknowledgement	International Science and Technology Center {[}3230]; Government of Sverdlovsk Region
Funding-Text	Manuscript received March 12, 2010; accepted March 14, 2010. Date of publication June 10, 2010; date of current version September 15, 2010. This work was supported in part by a grant from the International Science and Technology Center under Project 3230 and in part by the Government of Sverdlovsk Region under Project ``Nanotechnologies in bio- and chemical sensor controls for monitoring an environment and health of the person.{''} The associate editor coordinating the review of this paper and approving it for publication was Prof. Evgeny Katz.
Number-of-Cited-References	22
Usage-Count-Last-180-days	1
Usage-Count-Since-2013	13
Journal-ISO	IEEE Sens. J.
Doc-Delivery-Number	668SV