Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver's Effects with a Complex of Innocuous Bioprotectors / Katsnelson Boris A.,Privalova Larisa I.,Gurvich Vladimir B.,Makeyev Oleg H.,Shur Vladimir Ya.,Beikin Yakov B.,Sutunkova Marina P.,Kireyeva Ekaterina P.,Minigalieva Ilzira A.,Loginova Nadezhda V.,Vasilyeva Marina S.,Korotkov Artem V.,Shuman Eugene A.,Vlasova Larisa A.,Shishkina Ekaterina V.,Tyurnina Anast

ISSN/EISSN:

1422-0067 / нет данных

Type:

Article

Abstract:

Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat's pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a ``bioprotective complex{''} (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS.

Author keywords:

nanosilver; nanogold; bronchoalveolar lavage; subchronic toxicity; genotoxicity; bioprotectors QUARTZ DUST; OXIDATIVE STRESS; INTRATRACHEAL INSTILLATION; MULTICOMPARTMENTAL MODEL; CYTO-TOXICITY; COMET ASSAY; CELLS; GENOTOXICITY; RATS; LUNG

DOI:

10.3390/ijms14022449

Web of Science ID:

ISI:000315397900012

Соавторы в МНС:

Другие поля

Поле	Значение
Month	FEB
Publisher	MDPI AG
Address	POSTFACH, CH-4005 BASEL, SWITZERLAND
Language	English
Keywords-Plus	QUARTZ DUST; OXIDATIVE STRESS; INTRATRACHEAL INSTILLATION; MULTICOMPARTMENTAL MODEL; CYTO-TOXICITY; COMET ASSAY; CELLS; GENOTOXICITY; RATS; LUNG
Research-Areas	Biochemistry \& Molecular Biology; Chemistry
Web-of-Science-Categories	Biochemistry \& Molecular Biology; Chemistry, Multidisciplinary
Author-Email	bkaznelson@ymrc.ru privalovali@yahoo.com gurvich@ymrc.ru ommt305@mail.ru vladimir.shur@usu.ru kdc\_boss@mail.ru marinasutunkova@yandex.ru katerinakir@yandex.ru ilzira-minigalieva@yandex.ru tushkanN@yandex.ru myshkalev@rambler.ru akorotkov64@mail.ru eshuman@gmail.com larus-a@rambler.ru ekaterina.shishkina@labfer.usu.ru anastasiya.tyurnina@labfer.usu.ru roman.kozin@labfer.usu.ru valamina2012@yandex.ru ekb-lem@mail.ru tulakina@inbox.ru
ResearcherID-Numbers	Makeyev, Oleg/G-5511-2017 Shishkina, Ekaterina/I-1829-2017 Shur, Vladimir/J-9078-2015
ORCID-Numbers	Shishkina, Ekaterina/0000-0002-2574-7472
Number-of-Cited-References	72
Usage-Count-Last-180-days	5
Usage-Count-Since-2013	51
Journal-ISO	Int. J. Mol. Sci.
Doc-Delivery-Number	096IR