Subchronic Toxicity of Copper Oxide Nanoparticles and Its Attenuation with the Help of a Combination of Bioprotectors / Privalova Larisa I.,Katsnelson Boris A.,Loginova Nadezhda V.,Gurvich Vladimir B.,Shur Vladimir Y.,Valamina Irene E.,Makeyev Oleg H.,Sutunkova Marina P.,Minigalieva Ilzira A.,Kireyeva Ekaterina P.,Rusakov Vadim O.,Tyurnina Anastasia E.,Kozin Roman V.,Meshtcheryakova Ekaterina Y.,Korotkov Artem V.,Shuman Eugene A.,Zvereva Anastasia E.,Kostykova Svetlana V. // INTERNATIONAL JOURNA

ISSN/EISSN:
1422-0067 / нет данных
Type:
Article
Abstract:
In the copper metallurgy workplace air is polluted with condensation aerosols, which a significant fraction of is presented by copper oxide particles <100 nm. In the scientific literature, there is a lack of their in vivo toxicity characterization and virtually no attempts of enhancing organism's resistance to their impact. A stable suspension of copper oxide particles with mean (+/- SD) diameter 20 +/- 10 nm was prepared by laser ablation of pure copper in water. It was being injected intraperitoneally to rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week up to 19 injections. In parallel, another group of rats was so injected with the same suspension against the background of oral administration of a. bio-protective complex. (BPC) comprising pectin, a multivitamin-multimineral preparation, some amino acids and fish oil rich in omega-3 PUFA. After the termination of injections, many functional and biochemical indices for the organism's status, as well as pathological changes of liver, spleen, kidneys, and brain microscopic structure were evaluated for signs of toxicity. In the same organs we have measured accumulation of copper while their cells were used for performing the Random Amplification of Polymorphic DNA (RAPD) test for DNA fragmentation. The same features were assessed in control rats infected intraperitoneally with water with or without administration of the BPC. The copper oxide nanoparticles proved adversely bio-active in all respects considered in this study, their active in vivo solubilization in biological fluids playing presumably an important role in both toxicokinetics and toxicodynamics. The BPC proposed and tested by us attenuated systemic and target organs toxicity, as well as genotoxicity of this substance. Judging by experimental data obtained in this investigation, occupational exposures to nano-scale copper oxide particles can present a significant health risk while the further search for its management with the help of innocuous bioprotectors seems to be justified.
Author keywords:
copper oxide nanoparticles; subchronic toxicity; genotoxicity; bioprotectors IN-VIVO; CUO NANOPARTICLES; INHALED NANOPARTICLES; SILVER NANOPARTICLES; GENOTOXICITY; MECHANISMS; RATS; NEPHROTOXICITY; HEPATOCYTES; EXPRESSION
DOI:
10.3390/ijms150712379
Web of Science ID:
ISI:000340038500073
Соавторы в МНС:
Другие поля
Поле Значение
Month JUL
Publisher MDPI AG
Address POSTFACH, CH-4005 BASEL, SWITZERLAND
Language English
Keywords-Plus IN-VIVO; CUO NANOPARTICLES; INHALED NANOPARTICLES; SILVER NANOPARTICLES; GENOTOXICITY; MECHANISMS; RATS; NEPHROTOXICITY; HEPATOCYTES; EXPRESSION
Research-Areas Biochemistry \& Molecular Biology; Chemistry
Web-of-Science-Categories Biochemistry \& Molecular Biology; Chemistry, Multidisciplinary
Author-Email privalovali@yahoo.com bkaznelson@etel.ru tushkanN@yandex.ru Gurvich@ymrc.ru vladimir.shur@usu.ru ivalamina@mail.ru ommt305@mail.ru marinasutunkova@yandex.ru minigalieva@yandex.ru katerinakir@yandex.ru honored\_sci@yahoo.com anastasiya.tyurnina@labfer.usu.ru roman.kozin@labfer.usu.ru katusha-ugma@rambler.ru akorotkov64@mail.ru eshuman@gmail.com dor\_3110@mail.ru svkostyukova@mail.ru
ResearcherID-Numbers Makeyev, Oleg/G-5511-2017 Shur, Vladimir/J-9078-2015
Number-of-Cited-References 51
Usage-Count-Last-180-days 5
Usage-Count-Since-2013 47
Journal-ISO Int. J. Mol. Sci.
Doc-Delivery-Number AM7ID