Mossbauer spectroscopy of the iron cores in human liver ferritin, ferritin in normal human spleen and ferritin in spleen from patient with primary myelofibrosis: preliminary results of comparative analysis / Oshtrakh M. I.,Alenkina I. V.,Vinogradov A. V.,Konstantinova T. S.,Kuzmann E.,Semionkin V. A. // BIOMETALS. - 2013. - V. 26, l. 2. - P. 229-239.

ISSN/EISSN:

0966-0844 / 1572-8773

Type:

Article

Abstract:

Comparative study of human liver ferritin and spleen tissues from healthy human and patient with primary myelofibrosis was carried out using Mossbauer spectroscopy with a high velocity resolution at 295 and 90 K and with a low velocity resolution at 20 K. The results obtained demonstrated that the iron content in patient's spleen in the form of iron storage proteins was about ten times larger than that in normal tissue. However, in the case of patient with primary myelofibrosis the magnetic anisotropy energy barrier differed from that in normal case and, probably, the iron core size was supposed to be slightly larger than that in both normal spleen tissue and normal human liver ferritin in contrast to well-known data for iron overload in patients with thalassemia accompanied by the iron-core size increase. Therefore, the iron overload in the case of patient with primary myelofibrosis may be related to increase in the ferritin content mainly. It was also found that Mossbauer hyperfine parameters for normal and patient's spleen and normal human liver ferritin demonstrated some small differences related, probably, to some small structural variations in the ferritin iron cores of patient's spleen.

Author keywords:

Iron storage proteins; Spleen tissues; Mossbauer spectroscopy; Primary myelofibrosis HIGH-VELOCITY RESOLUTION; OVERLOAD DISEASES; CHICKEN LIVER; MAGNETIC-PROPERTIES; MYELOID METAPLASIA; OXIDE DEPOSITS; TISSUES; HEMOSIDERIN; DIAGNOSIS; PROTEINS

DOI:

10.1007/s10534-012-9602-2

Web of Science ID:

ISI:000319802100003

Соавторы в МНС:

Другие поля

Поле	Значение
Month	APR
Publisher	SPRINGER
Address	VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
Language	English
EISSN	1572-8773
Keywords-Plus	HIGH-VELOCITY RESOLUTION; OVERLOAD DISEASES; CHICKEN LIVER; MAGNETIC-PROPERTIES; MYELOID METAPLASIA; OXIDE DEPOSITS; TISSUES; HEMOSIDERIN; DIAGNOSIS; PROTEINS
Research-Areas	Biochemistry \& Molecular Biology
Web-of-Science-Categories	Biochemistry \& Molecular Biology
Author-Email	oshtrakh@mail.utnet.ru
ResearcherID-Numbers	Oshtrakh, Mikhail/L-8342-2016 Kuzmann, Erno/I-1789-2017
ORCID-Numbers	Oshtrakh, Mikhail/0000-0002-2937-5194 Kuzmann, Erno/0000-0002-0183-6649
Funding-Acknowledgement	Ministry of Science and Education of Russian Federation; Ural Federal University
Funding-Text	The authors wish to thank Prof. P. G. Prokopenko (Department of Biochemistry, Russian State Medical University, Moscow) for the gift of human liver ferritin and Dr. S. E. Ioshchenko for histochemical analysis and optical micrographs (Sverdlovsk Regional Bureau of Morbid Anatomy, Ekaterinburg). Many thanks to Dr. Peter Nemeth (Chemical Research Center, HAS, Budapest) for his electron microscopy images. This work was supported by the basic financing from the Ministry of Science and Education of Russian Federation. I. V. A. is supported in part by the Ural Federal University development program for support of young scientists.
Number-of-Cited-References	31
Usage-Count-Last-180-days	2
Usage-Count-Since-2013	13
Journal-ISO	Biometals
Doc-Delivery-Number	156DU