Characterization of the heme iron in pyridoxylated hemoglobin cross-linked by glutaraldehyde using Mossbauer spectroscopy / Oshtrakh MI,Milder OB,Semionkin VA,Berkovsky AL,Azhigirova MA,Vyazova EP // INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - 2000. - V. 28, l. 1. - P. 51-58.

ISSN/EISSN:

0141-8130 / 1879-0003

Type:

Article

Abstract:

The heme iron in human adult hemoglobin modified by both pyridoxal-5'-phosphate and glutaraldehyde was characterized by Mossbauer spectroscopy and compared with non-modified hemoglobin. Mossbauer spectra of the samples were measured at 87 and 295 K (lyophilized form) and at 87 K (frozen solution). The values of quadrupole splitting for the oxy-form of modified hemoglobin were found to be lower than those of the oxy-form of hemoglobin without modifications in lyophilized form and frozen solution, respectively. On the other hand, the values of quadrupole splitting for the deoxy-form of modified and non-modified hemoglobins in frozen solution were the same. The Mossbauer spectra of the oxy-form of modified hemoglobin were also analyzed in terms of the heme iron non-equivalence in alpha- and beta-subunits of tetramer. The differences of the tendencies of temperature dependencies of quadrupole splitting for the oxy-form of modified and non-modified hemoglobins in lyophilized form were shown. These results indicated that the heme iron electronic structure and stereochemistry were changed in the oxy-form of pyridoxylated hemoglobin cross-linked by glutaraldehyde. (C) 2000 Elsevier Science B.V. All rights reserved.

Author keywords:

hemoglobin; pyridoxal-5 `-phosphate; glutaraldehyde; Mossbauer spectroscopy; heme iron electronic structure and stereochemistry RED-BLOOD-CELLS; ELECTRONIC-STRUCTURE; MOLECULAR-STRUCTURE; SPECTRA; OXYHEMOGLOBIN; STEREOCHEMISTRY; MYOGLOBIN; SUBUNITS; FEATURES; SITE

DOI:

10.1016/S0141-8130(00)00142-2

Web of Science ID:

ISI:000089933100006

Соавторы в МНС:

Другие поля

Поле	Значение
Month	OCT 10
Publisher	ELSEVIER SCIENCE BV
Address	PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Language	English
EISSN	1879-0003
Keywords-Plus	RED-BLOOD-CELLS; ELECTRONIC-STRUCTURE; MOLECULAR-STRUCTURE; SPECTRA; OXYHEMOGLOBIN; STEREOCHEMISTRY; MYOGLOBIN; SUBUNITS; FEATURES; SITE
Research-Areas	Biochemistry \& Molecular Biology; Chemistry; Polymer Science
Web-of-Science-Categories	Biochemistry \& Molecular Biology; Chemistry, Applied; Polymer Science
Author-Email	oshtrakh@soek.erl.e-burg.ru
ResearcherID-Numbers	Oshtrakh, Mikhail/L-8342-2016
ORCID-Numbers	Oshtrakh, Mikhail/0000-0002-2937-5194
Number-of-Cited-References	32
Usage-Count-Last-180-days	1
Usage-Count-Since-2013	2
Journal-ISO	Int. J. Biol. Macromol.
Doc-Delivery-Number	365DL