COMPARISON OF HUMAN OXYHEMOGLOBIN IN LYOPHILIZED FORM, RED-BLOOD-CELLS, AND CONCENTRATED-SOLUTION - THE FEATURES OF MOSSBAUER-SPECTRA AND HEME IRON STEREOCHEMISTRY / OSHTRAKH MI // JOURNAL OF INORGANIC BIOCHEMISTRY. - 1994. - V. 56, l. 4. - P. 221-231.

ISSN/EISSN:

0162-0134 / нет данных

Type:

Article

Abstract:

Mossbauer spectra of human oxyhemoglobin in red blood cells, concentrated solution, and lyophilized form were measured at 87 K. Additionally, Mossbauer spectra of lyophilized oxyhemoglobin were measured at 295 K. The values of quadrupole splitting appeared to be the same for oxyhemoglobin in red blood cells and concentrated solution and slightly lower than those of oxyhemoglobin in lyophilized form. The asymmetry of the Mossbauer adsorption line shapes previously observed for oxyhemoglobin in red blood cells were also found for oxyhemoglobin in concentrated solution. These Mossbauer spectra were better fitted using two quadrupole split doublets with almost equal areas. In contrast, Mossbauer spectra of lyophilized oxyhemoglobin were symmetrical and satisfactorily fitted with one quadrupole split doublet. However, these spectra were also fitted with two quadrupole split doublets with equal areas. The variations of the absorption line shapes and parameters of oxyhemoglobin Mossbauer spectra were analyzed in terms of stereochemical differences of the heme iron and Fe(II)-O-2 bond in alpha- and beta-subunits of tetrameric oxyhemoglobin.

Author keywords:

FE-O2 BOND; SPECTROSCOPY; MODEL; HEMOGLOBIN; STATE; MYOGLOBIN; COMPLEXES; SUBUNITS; CASSCF

DOI:

10.1016/0162-0134(94)85102-6

Web of Science ID:

ISI:A1994PZ07500001

Соавторы в МНС:

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Другие поля

Поле	Значение
Month	DEC
Publisher	ELSEVIER SCIENCE PUBL CO INC
Address	655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010
Language	English
Keywords-Plus	FE-O2 BOND; SPECTROSCOPY; MODEL; HEMOGLOBIN; STATE; MYOGLOBIN; COMPLEXES; SUBUNITS; CASSCF
Research-Areas	Biochemistry \& Molecular Biology; Chemistry
Web-of-Science-Categories	Biochemistry \& Molecular Biology; Chemistry, Inorganic \& Nuclear
ResearcherID-Numbers	Oshtrakh, Mikhail/L-8342-2016
ORCID-Numbers	Oshtrakh, Mikhail/0000-0002-2937-5194
Number-of-Cited-References	32
Usage-Count-Since-2013	2
Journal-ISO	J. Inorg. Biochem.
Doc-Delivery-Number	PZ075