Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells / Vladimirov A. P.,Malygin A. S.,Mikhailova J. A.,Borodin E. M.,Bakharev A. A.,Poryvayeva A. P. // . - 2014. - V. 536, l. .

ISSN/EISSN:

1742-6588 / нет данных

Type:

Proceedings Paper

Abstract:

Earlier we reported developing a speckle interferometry technique and a device designed to assess the metabolic activity of a cell monolayer cultivated on a glass substrate. This paper aimed at upgrading the technique and studying its potential for real-time assessment of herpes virus development process. Speckle dynamics was recorded in the image plane of intact and virus-infected cell monolayer. HLE-3, L-41 and Vero cells were chosen as research targets. Herpes simplex virus-1-(HSV-1)-infected cell cultures were studied. For 24 h we recorded the digital value of optical signal I in one pixel and parameter eta characterizing change in the distribution of the optical signal on 10x10-pixel areas. The coefficient of multiple determination calculated by eta time dependences for three intact cell cultures equals 0.94. It was demonstrated that the activity parameters are significantly different for intact and virus-infected cells. The difference of eta value for intact and HSV-1-infected cells is detectable 10 minutes from the experiment start.

Author keywords:

нет данных

DOI:

10.1088/1742-6596/536/1/012012

Web of Science ID:

ISI:000346520400012

Соавторы в МНС:

Другие поля

Поле	Значение
Book-Group-Author	IOP
Booktitle	1ST INTERNATIONAL SCIENTIFIC SCHOOL ON METHODS OF DIGITAL IMAGE PROCESSING IN OPTICS AND PHOTONICS
Series	Journal of Physics Conference Series
Note	1st International Scientific School on Methods of Digital Image Processing in Optics and Photonics, St Petersburg, RUSSIA, APR 08-11, 2014
Publisher	IOP PUBLISHING LTD
Address	DIRAC HOUSE, TEMPLE BACK, BRISTOL BS1 6BE, ENGLAND
Language	English
Research-Areas	Optics; Physics
Web-of-Science-Categories	Optics; Physics, Applied
Author-Email	vap52@bk.ru
ResearcherID-Numbers	Mikhailova, Yuliya/L-3153-2015
Number-of-Cited-References	5
Usage-Count-Last-180-days	2
Usage-Count-Since-2013	15
Doc-Delivery-Number	BB8GF