A speckle-interferometric device for studying the cell biological activity / Malygin A. S.,Bebenina N. V.,Vladimirov A. P.,Mikitas K. N.,Baharev A. A. // INSTRUMENTS AND EXPERIMENTAL TECHNIQUES. - 2012. - V. 55, l. 3. - P. 415-418.

ISSN/EISSN:

0020-4412 / нет данных

Type:

Article

Abstract:

A modernized device intended for diagnosing the temperature-dependent activity of cells is described. The activity of cells was determined by finding the correlation coefficient of optical signals in speckle patterns recorded at different moments of time. It is shown that, as the temperature increases from 26 to 36A degrees C, variations in the optical paths in cells increase from 16 to 26 nm. This is probably associated with the physiological activity of cells-trapping of large molecules or their conglomerates. In contrast to the previous variant of the device design, instead of a lipid membrane, a cell monolayer was used, which was cultivated on a plane-parallel glass plate. In order to reduce variations in the optical path lengths in a nutrient solution, a 1.7-mm-thick plane-parallel glass plate was additionally inserted into the cuvette. The modernization of the device also includes heating of the cuvette with hot air flows.

Author keywords:

нет данных

DOI:

10.1134/S0020441212020091

Web of Science ID:

ISI:000304877000017

Соавторы в МНС:

Другие поля

Поле	Значение
Month	MAY
Publisher	MAIK NAUKA/INTERPERIODICA/SPRINGER
Address	233 SPRING ST, NEW YORK, NY 10013-1578 USA
Language	English
Research-Areas	Engineering; Instruments \& Instrumentation
Web-of-Science-Categories	Engineering, Multidisciplinary; Instruments \& Instrumentation
Author-Email	somt@bk.ru
ResearcherID-Numbers	Vladimirov, Alexander/K-2967-2012
ORCID-Numbers	Vladimirov, Alexander/0000-0001-6343-9772
Number-of-Cited-References	11
Usage-Count-Last-180-days	4
Usage-Count-Since-2013	26
Journal-ISO	Instrum. Exp. Tech.
Doc-Delivery-Number	953NY