Applicaton of speckle dynamics for studies of cell metabolism / Vladimirov A.P., Baharev A.A., Malygin A.S., Mikhailova J.A., Novoselova I.A., Yakin D.I. // Proceedings of SPIE - The International Society for Optical Engineering. - 2015. - V. 9529, l. .

ISSN:

0277786X

Type:

Conference Paper

Abstract:

Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. The target of the research was cells from L-41 culture placed onto a special optical tray with a nutrient solution immediately after defrosting. Time-average value T of digital radiation intensity in the TV camera pixels I (1) and the correlation coefficient of two digital images η (2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments. It is shown that in the area containing hundreds of cells dependence η(t) levels off, which indicate stationarity of random value I∼. Features of η(t) dependences obtained by averaging over large and small cell numbers image are discussed. Using the data obtained, we formulated recommendations on selecting area sizes for averaging physical values to study various processes occurring in cells. © 2015 SPIE.

Author keywords:

cellular metabolism; dynamic speckle interferometry; herpes virus; speckles

Index keywords:

Cells; Cytology; Interferometry; Metabolism; Physiology; Viruses; Cellular metabolism; Correlation coefficient; Dynamic speckle; Metabolic activity; Nutrient solution; Quantitative evaluation; Radiati

DOI:

10.1117/12.2184605

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938824105&doi=10.1117%2f12.2184605&partnerID=40&md5=22ed4b87e6f504ae25217c5e0f819ae0

Соавторы в МНС:

Другие поля

Поле	Значение
Art. No.	95291F
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938824105&doi=10.1117%2f12.2184605&partnerID=40&md5=22ed4b87e6f504ae25217c5e0f819ae0
Affiliations	Ural Federal University, Yekaterinburg, Russian Federation; Research Institute of Viral Infections, Yekaterinburg, Russian Federation
Author Keywords	cellular metabolism; dynamic speckle interferometry; herpes virus; speckles
References	Severin, E.S., (2003) Biochemistry: Textbook for High Schools, 779. , GEOTAR-Media, Moscow; Rabal, H.J., Braga, R.A., (2008) Dynamic Laser Speckle and Applications, , CRC Press; Shebalin, A., Laser medical diagnostics of the organism biospeckle skin (2008) PHOTONICS, 1, pp. 14-18; Vladimirov, A.P., Druzhinin, A.V., Malygin, A.S., Mikitas, K.N., Theory and calibration of speckle dynamics of phase object (2012) Proc. SPIE, Saratov Fall Meeting 2011: Optical Technologies in Biophysics and Medicine, 13, p. 8337; Malygin, A.S., Bebenina, N.V., Vladimirov, A.P., A speckle-interferometric device for studying the cell biological activity (2012) Instrum. Exp. Tech., 55 (3), pp. 415-418; Vladimirov, A.P., Dynamic speckle-interferometry of microscopic processes in thin biological objects (2015) Radiophysics and Quantum Electronics, 57 (8-9), pp. 564-576; Vladimirov, A.P., Malygin, A.S., Mikhailova Yu., A., Using speckle dynamics for comparison of the metabolic activity of different cell cultures (2015) Radiophysics and Quantum Electronics, 57 (8-9), pp. 584-588; Vladimirov, A.P., Malygin, A.S., Mikhailova Yu., A., Retrofit device for real-time evaluation of metabolic activity in herpes virus infected cell cultures (2014) Biomedical Engineering, 48 (4), pp. 178-181
Editors	Grilli S.Ferraro P.Ritsch-Marte M.Stifter D.
Sponsors	The Society of Photo-Optical Instrumentation Engineers (SPIE)
Publisher	SPIE
Conference name	Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Conference date	22 June 2015 through 24 June 2015
Conference code	113145
ISBN	9781628416893
CODEN	PSISD
Language of Original Document	English
Abbreviated Source Title	Proc SPIE Int Soc Opt Eng
Source	Scopus