Dynamic speckle-interferometer for intracellular processes analyses at high optical magnification / Baharev A.A., Vladimirov A.P., Malygin A.S., Mikhailova Y.A., Novoselova I.A., Yakin D.I., Druzhinin A.V. // Proceedings of SPIE - The International Society for Optical Engineering. - 2015. - V. 9529, l. .

ISSN:
0277786X
Type:
Conference Paper
Abstract:
At present work dynamic of biospeckles is used for studying processes occurring in cells which arranged in the one layer. The basis of many diseases is changes in the structural and functional properties of the molecular cells components as caused by the influence of external factors and internal functional disorders. Purpose of work is approbation of speckle-interferometer designed for the analysis of cellular metabolism in individual cells. As a parameter, characterizing the metabolic activity of cells used the value of the correlation coefficient (η) of optical signals proportional to the radiation intensity I, recorded at two points in time t. At 320x magnification for the cell diameter of 20 microns value η can be determined in the area size of 6 microns. © 2015 SPIE.
Author keywords:
auto-correlation function; cell; cells activity; cells cultures; human diseases; interferometry; intracellular processes; laser; metabolism; radiation intensity; Speckle
Index keywords:
Cytology; Interferometers; Interferometry; Lasers; Metabolism; Optical correlation; Physiology; Speckle; Autocorrelation functions; Cellular metabolism; Correlation coefficient; Functional properties;
DOI:
10.1117/12.2184622
Смотреть в Scopus:
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938868963&doi=10.1117%2f12.2184622&partnerID=40&md5=4362af26d1dee4c0202f6e27d72bc2c2
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Art. No. 95291G
Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938868963&doi=10.1117%2f12.2184622&partnerID=40&md5=4362af26d1dee4c0202f6e27d72bc2c2
Affiliations Laboratory of Cell Cultures, Research Institute of Virus Infections, str. Letnyaya 23, Yekaterinburg, Russian Federation; Department of Physical Control Methods and Devices, Institute of Physical Engineering, Ural Federal University, str. Mira 19, Yekaterinburg, Russian Federation; Research Institute of Virus Infections, str. Letnyaya 23, Yekaterinburg, Russian Federation; Department of Experimental Physics, Institute of Physical Engineering, Ural Federal University, str. Mira 19, Yekaterinburg, Russian Federation; Institute of Virus Infections, str. Letnyaya 23, Yekaterinburg, Russian Federation; Institute of Metal Physics, str. S. Kovalevskoy 15, Yekaterinburg, Russian Federation
Author Keywords auto-correlation function; cell; cells activity; cells cultures; human diseases; interferometry; intracellular processes; laser; metabolism; radiation intensity; Speckle
References Franson, M., Optics of Speckles, 171, p. 1980. , M.: Mir; Tuchin, V.V., (2010) Lasers and Optical Fibers in Biomedical Research. 2nd Ed. Rev. and Add., p. 488. , M.: FIZMATLIT; Tuchinsky, V.P., Coherent phase microscopy of intracellular processes (2001) Advances in Physical Sciences, 171, pp. 649-661; Zimnyakov, D.A., Tuchin, V.V., Utc, S.R., The study of the statistical properties of partially developed speckle fields in relation to the diagnosis of structural changes of human skin (1994) Optics and Spectroscopy. JNo5., pp. 13-16; Vladimirov, A.P., Zimin, A.L., Mikushin, V.I., Kohan, M.M., Kuklin, I.A., Kononenko, E.V., Application technique of counting the number of speckles flicker to study the biological activity of the skin (2000) Letters to the Journal of Technical Physics., 26, pp. 20-24; Akchurin, G.G., Bakutkin, V.V., Radchenko, E.Y., Tuchin, V.V., Laser speckle interferometry and the ability to determine the retinal visual acuity in cataract.'' (2002) Biomedical Technology and Electronics. JNo1, pp. 19-27; Dubnishchev, Y.N., Arbuzov, V.A., Belousov, P.P., Belousov, P.Y., Optical Methods of Research Streams, 418, p. 2003. , Ed. Acad. RAS V.E. Nakoryakova Novosibirsk: Siberian university publishing house; Poryvaeva, A.P., Nekrasov, T.S., Cell cultures in complex diagnostics of herpesvirus infections 6th Regional Scientific and Practical Conference "prospects for the Use in Medical Practice New Immunobiological Preparations Derived from Cell Cultures, , on 30-31 May 2012 Ekaterinburg. Proceedings 2012; Vladimirov, A.P., Druzhinin, A.V., Malygin, A.S., Mikitas, K.N., Theory and calibration of speckle dynamics of phase Object Proc. of SPIE, 8337, pp. 83370C001-83370C159; Malygin, A.S., Bebenina, N.V., Vladimirov, A.P., A speckle-interferometric device for studying the cell biological activity (2012) Instrum. Exp. Tech., 55 (3), pp. 415-418; Bebenina, N.V., Application of the Dynamic Speckle Interferometry to Study the Metabolic Activity of Cells in Experiments in Vitro, 76, p. 2012. , Master's thesis.-Ekaterinburg; Mikhailova, Y.A., The use of dynamic speckle interferometry for analyzing the metabolic activity of cultured cells: Final qualifying work Ekaterinburg, 66, p. 2013; Patent JN° 2148644 RF. N.P Glinskikh, V.N. Avdeev, F.YA Zussman and others; Vladimirov, A.P., Dynamic speckle-interferometry of microscopic processes in a thin biological objects (2015) Radiophysics and Quantum Electronics, 57 (8), pp. 564-657
Editors Grilli S.Ferraro P.Ritsch-Marte M.Stifter D.
Sponsors The Society of Photo-Optical Instrumentation Engineers (SPIE)
Publisher SPIE
Conference name Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Conference date 22 June 2015 through 24 June 2015
Conference code 113145
ISBN 9781628416893
CODEN PSISD
Language of Original Document English
Abbreviated Source Title Proc SPIE Int Soc Opt Eng
Source Scopus