Dynamic speckle interferometry of intracellular processes: Theory and features of application / Vladimirov A.P. // Proceedings of SPIE - The International Society for Optical Engineering. - 2015. - V. 9529, l. .

ISSN:

0277786X

Type:

Conference Paper

Abstract:

The purpose of the work was the link establishment between the parameters characterizing the change phase difference of the wave pairs, passing through the cell and the parameters of the dynamics of speckle. It was assumed that the change in wave phases is caused by microscopic and macroscopic processes in the cell. Optical system is considered in theoretical part. It is consist of the source of coherent radiation, thin transparent diffuser, thin transparent biological object and thin lens which forms an image of the object. It is assumed that the diffuser consists of randomly located scattering point centers. Also, it is assumed that optical path length of single wave: a) changes with time t randomly due to the microscopic processes on the structure level, and b) are determined for the shape change (deformation) of the thin object. An expression for the time autocorrelation function η(t) of the radiation intensity I∼ at some point in the observation plane is given. Function η(t) type for stationary and non-stationary change of the path difference of the waves is discussed. Typical experimental dependences I∼(t) and η(t) are shown. It is shown that a focused selection of averaging time T allows creating the conditions for obtaining reproducible results, highlighting and analyzing processes occurring in cells at different velocities. © 2015 SPIE.

Author keywords:

cell biology; metabolic process; single sell; speckle; speckle dynamics

Index keywords:

Autocorrelation; Cells; Cytology; Optical systems; Cell biology; Intracellular process; Metabolic process; Optical path lengths; Parameters characterizing; single sell; Speckle dynamics; Time autocorr

DOI:

10.1117/12.2184567

Смотреть в Scopus:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938820589&doi=10.1117%2f12.2184567&partnerID=40&md5=e2c05ab16d776adf98b052d273631eb0

Соавторы в МНС:

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Другие поля

Поле	Значение
Art. No.	95290Y
Link	https://www.scopus.com/inward/record.uri?eid=2-s2.0-84938820589&doi=10.1117%2f12.2184567&partnerID=40&md5=e2c05ab16d776adf98b052d273631eb0
Affiliations	Ural Federal University, Yekaterinburg, Russian Federation; Research Institute of Viral Infections, Yekaterinburg, Russian Federation
Author Keywords	cell biology; metabolic process; single sell; speckle; speckle dynamics
References	Rabal, H.J., Braga, R.A., (2008) Dynamic Laser Speckle and Applications, , CRC Press; Briers, J.D., Laser speckle contrast imaging for measuring blood flow (2007) Optica Applicata, 37 (1-2), pp. 139-152; Vladimirov, A.P., Druzhinin, A.V., Malygin, A.S., Mikitas, K.N., Theory and calibration of speckle dynamics of phase object (2012) Proc. SPIE, 8337; Malygin, S.A., Vladimirov, A.P., Bebenina, N.V., Mikitas, K.N., Baharev, A.A., A speckle interferometric device for studying the cell biological activity (2012) Instruments and Experimental Techniques, 55 (3), pp. 415-418; Vladimirov, A.P., Dynamic speckle-interferometry of microscopic processes in thin biological objects (2015) Radiophysics and Quantum Electronics, 57 (8), pp. 564-576
Correspondence Address	Vladimirov, A.P.; Ural Federal UniversityRussian Federation
Editors	Grilli S.Ferraro P.Ritsch-Marte M.Stifter D.
Sponsors	The Society of Photo-Optical Instrumentation Engineers (SPIE)
Publisher	SPIE
Conference name	Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II
Conference date	22 June 2015 through 24 June 2015
Conference code	113145
ISBN	9781628416893
CODEN	PSISD
Language of Original Document	English
Abbreviated Source Title	Proc SPIE Int Soc Opt Eng
Source	Scopus