Application of dynamic speckle interferometry for diagnosis of metabolism change in cultured cells / Vladimirov A. P.,Mikhaylova J. A.,Baharev A. A.,Malygin A. S.,Novosyolova I. A.,Yakin D. I. // . - 2015. - V. 9660, l. .

ISSN/EISSN:

0277-786X / нет данных

Type:

Proceedings Paper

Abstract:

Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. Defrosted L-41 cells precipitated on a glass substrate were the target of the research. Time-average value T of digital radiation intensity in the TV camera pixels I-similar to (1) and the correlation coefficient of two digital images eta(2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments.

Author keywords:

speckles; dynamic speckle interferometry; cellular metabolism; herpes virus BIOLOGICAL-ACTIVITY; LASER SPECKLE; FLOW

DOI:

10.1117/12.2196090

Web of Science ID:

ISI:000360300500040

Соавторы в МНС:

Другие поля

Поле	Значение
Editor	Santoyo, FM and Mendez, ER
Booktitle	SPECKLE 2015: VI INTERNATIONAL CONFERENCE ON SPECKLE METROLOGY
Series	Proceedings of SPIE
Note	6th International Conference on Speckle Metrology (SPECKLE), Guanajuato, MEXICO, AUG 24-26, 2015
Organization	Centro Investigaciones Optica A C; Centro Investigac Cientifica \& Educac Super Ensenada; Consejo Nacl Ciencia \& Tecnologia; Consejo Ciencia \& Tecnologia Estado Guanajuato; Univ Guanajuato; SPIE
Publisher	SPIE-INT SOC OPTICAL ENGINEERING
Address	1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Language	English
Article-Number	UNSP 96601M
ISBN	978-1-62841-878-1
Keywords-Plus	BIOLOGICAL-ACTIVITY; LASER SPECKLE; FLOW
Research-Areas	Optics; Spectroscopy
Web-of-Science-Categories	Optics; Spectroscopy
ResearcherID-Numbers	Mikhailova, Yuliya/L-3153-2015
Number-of-Cited-References	13
Usage-Count-Last-180-days	6
Usage-Count-Since-2013	12
Doc-Delivery-Number	BD3YA