Dynamic speckle interferometry of intracellular processes: theory and features of application / Vladimirov A. P. // . - 2015. - V. 9529, l. .

ISSN/EISSN:

0277-786X / нет данных

Type:

Proceedings Paper

Abstract:

The purpose of the work was the link establishment between the parameters characterizing the change phase difference of the wave pairs, passing through the cell and the parameters of the dynamics of speckle. It was assumed that the change in wave phases is caused by microscopic and macroscopic processes in the cell. Optical system is considered in theoretical part. It is consist of the source of coherent radiation, thin transparent diffuser, thin transparent biological object and thin lens which forms an image of the object. It is assumed that the diffuser consists of randomly located scattering point centers. Also, it is assumed that optical path length of single wave: a) changes with time t randomly due to the microscopic processes on the structure level, and b) are determined for the shape change (deformation) of the thin object. An expression for the time autocorrelation function eta(t) of the radiation intensity (I) over tilde at some point in the observation plane is given. Function eta(t) type for stationary and non-stationary change of the path difference of the waves is discussed. Typical experimental dependences (I) over tilde (t) and eta(t) are shown. It is shown that a focused selection of averaging time T allows creating the conditions for obtaining reproducible results, highlighting and analyzing processes occurring in cells at different velocities.

Author keywords:

speckle; speckle dynamics; cell biology; single sell; metabolic process

DOI:

10.1117/12.2184567

Web of Science ID:

ISI:000358138000022

Соавторы в МНС:

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Другие поля

Поле	Значение
Editor	Ferraro, P and Grilli, S and RitschMarte, M and Stifter, D
Booktitle	OPTICAL METHODS FOR INSPECTION, CHARACTERIZATION, AND IMAGING OF BIOMATERIALS II
Series	Proceedings of SPIE
Note	Conference on Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, Munich, GERMANY, JUN 22-24, 2015
Organization	SPIE
Publisher	SPIE-INT SOC OPTICAL ENGINEERING
Address	1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Language	English
Article-Number	95290Y
ISBN	978-1-62841-689-3
Research-Areas	Materials Science; Microscopy; Optics; Radiology, Nuclear Medicine \& Medical Imaging
Web-of-Science-Categories	Materials Science, Multidisciplinary; Microscopy; Optics; Radiology, Nuclear Medicine \& Medical Imaging
ResearcherID-Numbers	Ferraro, Pietro/A-5288-2009
ORCID-Numbers	Ferraro, Pietro/0000-0002-0158-3856
Number-of-Cited-References	5
Doc-Delivery-Number	BD1LN