Application of speckle dynamics for studies of cell metabolism / Vladimirov A. P.,Baharev A. A.,Malygin A. S.,Mikhailova J. A.,Novoselova I. A.,Yakin D. I. // . - 2015. - V. 9529, l. .

ISSN/EISSN:

0277-786X / нет данных

Type:

Proceedings Paper

Abstract:

Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. The target of the research was cells from L-41 culture placed onto a special optical tray with a nutrient solution immediately after defrosting. Time-average value T of digital radiation intensity in the TV camera pixels I (1) and the correlation coefficient of two digital images eta(2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments. It is shown that in the area containing hundreds of cells dependence eta(t) levels off, which indicate stationarity of random value (I) over tilde. Features of eta(t) dependences obtained by averaging over large and small cell numbers image are discussed. Using the data obtained, we formulated recommendations on selecting area sizes for averaging physical values to study various processes occurring in cells.

Author keywords:

speckles; dynamic speckle interferometry; cellular metabolism; herpes virus

DOI:

10.1117/12.2184605

Web of Science ID:

ISI:000358138000033

Соавторы в МНС:

Другие поля

Поле	Значение
Editor	Ferraro, P and Grilli, S and RitschMarte, M and Stifter, D
Booktitle	OPTICAL METHODS FOR INSPECTION, CHARACTERIZATION, AND IMAGING OF BIOMATERIALS II
Series	Proceedings of SPIE
Note	Conference on Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, Munich, GERMANY, JUN 22-24, 2015
Organization	SPIE
Publisher	SPIE-INT SOC OPTICAL ENGINEERING
Address	1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
Language	English
Article-Number	95291F
ISBN	978-1-62841-689-3
Research-Areas	Materials Science; Microscopy; Optics; Radiology, Nuclear Medicine \& Medical Imaging
Web-of-Science-Categories	Materials Science, Multidisciplinary; Microscopy; Optics; Radiology, Nuclear Medicine \& Medical Imaging
ResearcherID-Numbers	Mikhailova, Yuliya/L-3153-2015
Number-of-Cited-References	8
Usage-Count-Last-180-days	7
Usage-Count-Since-2013	20
Doc-Delivery-Number	BD1LN