Application of dynamic speckle interferometry for diagnosis of metabolism change in cultured cells / Vladimirov A.P., Mikhailova J.A., Baharev A.A., Malygin A.S., Novosyolova I.A., Yakin D.I. // Proceedings of SPIE - The International Society for Optical Engineering. - 2015. - V. 9660, l. .

ISSN:
0277786X
Type:
Conference Paper
Abstract:
Previously, a speckle interferometry technique and a device that allows quantitative evaluation of the metabolic activity of cultured cells were theoretically grounded and successfully tested. A speckle time-averaging technique was proposed to separate and study the processes occurring in cells at various velocities. The objective of the present research was comparing the parameters of speckle dynamics used to evaluate cell metabolism and averaged in areas of various size. Areas inside the speckle image of a cell as well as areas of the image plane with various numbers of cells were averaging areas. Defrosted L-41 cells precipitated on a glass substrate were the target of the research. Time-average value T of digital radiation intensity in the TV camera pixels I∼ (1) and the correlation coefficient of two digital images no (2) were used as speckle field change parameters. The digital images corresponded to a single frame area at two time moments. © Copyright 2015 SPIE.
Author keywords:
cellular metabolism; dynamic speckle interferometry; herpes virus; Speckles
Index keywords:
Body fluids; Cells; Cytology; Holography; Interferometry; Metabolism; Physiology; Substrates; Units of measurement; Viruses; Cellular metabolism; Correlation coefficient; Dynamic speckle; Metabolic ac
DOI:
10.1117/12.2196090
Смотреть в Scopus:
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943549969&doi=10.1117%2f12.2196090&partnerID=40&md5=cf4830542620a7ae02e756bc30a32f01
Соавторы в МНС:
Другие поля
Поле Значение
Art. No. 96601M
Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943549969&doi=10.1117%2f12.2196090&partnerID=40&md5=cf4830542620a7ae02e756bc30a32f01
Affiliations Ural Federal University, Yekaterinburg, Russian Federation; Research Institute of Viral Infections, Yekaterinburg, Russian Federation
Author Keywords cellular metabolism; dynamic speckle interferometry; herpes virus; Speckles
References Severin, E.S., (2003) Biochemistry: Textbook for High Schools, 779. , GEOTAR-Media Moscow; Rabal, H.J., Braga, R.A., (2008) Dynamic Laser Speckle and Applications, , CRC Press; Cardoso, R.R., Costa, A.G., Nobre, C.M.B., Braga, R.A., Jr., Frequency signature of water activity by biospeckle laser (2011) Optics Communications, 284, pp. 2131-2136; Oulamara, A., Tribillon, G., Duvernoy, J., Biological activity measurement on botanical specimen surfaces using a temporal decorrelation effect of laser speckle (1989) Journal of Modern Optics, 36 (2), pp. 165-179; Vladimirov, A.P., Lisin, A.L., Mikushin, V.I., Kokhan, M.M., Kuklin, I.A., Kononenko, E.V., Biological activity of the human skin studied by method of speckle counting (2000) Technical Physics Letters, 26 (5), pp. 366-368; Fercher, A.F., Briers, J.D., Flow visualization by means of single-exposure speckle photography (1981) Optics Communications, 37 (5), pp. 326-330; Briers, J.D., Webster, S., Laser speckle contrast analysis (LASCA): A nonscanning, full-field technique for monitoring capillary blood flow (1996) Journal of Biomedical Optics, 1 (2), pp. 174-179; Briers, J.D., Laser speckle contrast imaging for measuring blood flow (2007) Optica Applicata, 37 (1-2), pp. 139-152; Vladimirov, A.P., Druzhinin, A.V., Malygin, A.S., Mikitas, K.N., Theory and calibration of speckle dynamics of phase object (2012) Proc. SPIE, Saratov Fall Meeting 2011: Optical Technologies in Biophysics and Medicine, 13, p. 8337; Malygin, A.S., Bebenina, N.V., Vladimirov, A.P., A speckle-interferometric device for studying the cell biological activity (2012) Instrum. Exp. Tech, 55 (3), pp. 415-418; Vladimirov, A.P., Dynamic speckle-interferometry of microscopic processes in thin biological objects (2015) Radiophysics and Quantum Electronics, 57 (8-9), pp. 564-576; Vladimirov, A.P., Malygin, A.S., Mikhailova Yu., A., Retrofit device for real-time evaluation of metabolic activity in herpes virus infected cell cultures (2014) Biomedical Engineering, 48 (4), pp. 178-181; Vladimirov, A.P., Malygin, A.S., Mikhailova Yu., A., Using speckle dynamics for comparison of the metabolic activity of different cell cultures (2015) Radiophysics and Quantum Electronics, 57 (8-9), pp. 584-588
Editors Santoyo F.M.Mendez E.R.
Sponsors Consejo de Ciencia y Tecnologia del Estado de Guanajuato;Consejo Nacional de Ciencia y Tecnologia;The Society of Photo-Optical Instrumentation Engineers (SPIE);Universidad de Guanajuato
Publisher SPIE
Conference name 6th International Conference on Speckle Metrology, SPECKLE 2015
Conference date 24 August 2015 through 26 August 2015
Conference code 114042
ISBN 9781628418781
CODEN PSISD
Language of Original Document English
Abbreviated Source Title Proc SPIE Int Soc Opt Eng
Source Scopus