Attenuation of Combined Nickel(II) Oxide and Manganese(II, III) Oxide Nanoparticles' Adverse Effects with a Complex of Bioprotectors / Minigalieva Ilzira A.,Katsnelson Boris A.,Privalova Larisa I.,Sutunkova Marina P.,Gurvich Vladimir B.,Shur Vladimir Y.,Shishkina Ekaterina V.,Valamina Irene E.,Makeyev Oleg H.,Panov Vladimir G.,Varaksin Anatoly N.,Grigoryeva Ekaterina V.,Meshtcheryakova Ekaterina Y. // INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - 2015. - V. 16, l. 9. - P. 22555-22583.

ISSN/EISSN:

1422-0067 / нет данных

Type:

Article

Abstract:

Stable suspensions of NiO and Mn3O4 nanoparticles (NPs) with a mean (+/- s.d.) diameter of 16.7 +/- 8.2 and 18.4 +/- 5.4 nm, respectively, purposefully prepared by laser ablation of 99.99\% pure nickel or manganese in de-ionized water, were repeatedly injected intraperitoneally (IP) to rats at a dose of 2.5 mg/kg 3 times a week up to 18 injections, either alone or in combination. A group of rats was injected with this combination with the background oral administration of a bio-protective complex (BPC) comprising pectin, vitamins A, C, E, glutamate, glycine, N-acetylcysteine, selenium, iodide and omega-3 PUFA, this composition having been chosen based on mechanistic considerations and previous experience. After the termination of injections, many functional and biochemical indices and histopathological features (with morphometric assessment) of the liver, spleen, kidneys and brain were evaluated for signs of toxicity. The Ni and Mn content of these organs was measured with the help of the atomic emission and electron paramagnetic resonance spectroscopies. We obtained blood leukocytes for performing the RAPD (Random Amplified Polymorphic DNA) test. Although both metallic NPs proved adversely bio-active in many respects considered in this study, Mn3O4-NPs were somewhat more noxious than NiO-NPs as concerns most of the non-specific toxicity manifestations and they induced more marked damage to neurons in the striatum and the hippocampus, which may be considered an experimental correlate of the manganese-induced Parkinsonism. The comparative solubility of the Mn3O4-NPs and NiO-NPs in a biological medium is discussed as one of the factors underlying the difference in their toxicokinetics and toxicities. The BPC has attenuated both the organ-systemic toxicity and the genotoxicity of Mn3O4-NPs in combination with NiO-NPs.

Author keywords:

nanoparticles; manganese(II; III) oxide; nickel(II) oxide; subchronic toxicity; bioprotectors SUBCHRONIC SYSTEMIC TOXICITY; IN-VIVO; INTRATRACHEAL INSTILLATION; URIC-ACID; EXPOSURE; CELLS; PHAGOCYTOSIS; GENOTOXICITY; INFLAMMATION; MECHANISMS

DOI:

10.3390/ijms160922555

Web of Science ID:

ISI:000364541000135

Соавторы в МНС:

Другие поля

Поле	Значение
Month	SEP
Publisher	MDPI AG
Address	POSTFACH, CH-4005 BASEL, SWITZERLAND
Language	English
Keywords-Plus	SUBCHRONIC SYSTEMIC TOXICITY; IN-VIVO; INTRATRACHEAL INSTILLATION; URIC-ACID; EXPOSURE; CELLS; PHAGOCYTOSIS; GENOTOXICITY; INFLAMMATION; MECHANISMS
Research-Areas	Biochemistry \& Molecular Biology; Chemistry
Web-of-Science-Categories	Biochemistry \& Molecular Biology; Chemistry, Multidisciplinary
Author-Email	ilzira-minigalieva@yandex.ru bkaznelson@etel.ru privalovali@yahoo.com marinasutunkova@yandex.ru gurvich@ymrc.ru vladimir.shur@urfu.ru ekaterina.shishkina@labfer.usu.ru ivalamina@mail.ru ommt305@mail.ru vpanov@ecko.uran.ru varaksin@ecko.uran.ru grigorieva@ymrc.ru katusha-ugma@rambler.ru
ResearcherID-Numbers	Makeyev, Oleg/G-5511-2017 Shishkina, Ekaterina/I-1829-2017 Shur, Vladimir/J-9078-2015
ORCID-Numbers	Shishkina, Ekaterina/0000-0002-2574-7472
Funding-Acknowledgement	Ministry of Education and Science of the Russian Federation {[}UID RFMEFI59414X0011]
Funding-Text	The equipment of the Ural Center for Shared Use ``Modern nanotechnology{''} (the Ural Federal University) was used. The research was ainancially supported in part by the Ministry of Education and Science of the Russian Federation (the grant UID RFMEFI59414X0011).
Number-of-Cited-References	46
Usage-Count-Last-180-days	7
Usage-Count-Since-2013	27
Journal-ISO	Int. J. Mol. Sci.
Doc-Delivery-Number	CV8MD