Amperometric detection of cholesterol using cobalt (II) chloride as an electrocatalyst in aprotic media / Kozitsina Alisa N.,Okhokhonin Andrei V.,Matern Anatoly I. // JOURNAL OF ELECTROANALYTICAL CHEMISTRY. - 2016. - V. 772, l. . - P. 89-95.

ISSN/EISSN:

1572-6657 / 1873-2569

Type:

Article

Abstract:

The determination of cholesterol concentration in the blood is an important characteristic of the state of health. Modern methods for the detection of cholesterol involve the use of enzymes or expensive and complicated equipment. In this study, an enzymeless electrochemical method for the quantitative determination of cholesterol using cobalt (II) chloride as an electrocatalyst has been proposed. An analytical signal was peak current of cobalt (II) chloride faradaic oxidation on a platinum disk electrode in acetonitrile media in the presence of cholesterol via linear sweep voltammetry. A possible path of the electrocatalytic oxidation of cholesterol on the electrode surface was proposed. The catalytic constant k(cat) and the Michaelis constant K-m calculated for the electrocatalytic oxidation process of cholesterol on the electrode surface are comparable in magnitude to the natural enzyme cholesterol oxidase, indicating the high efficiency of CoCl2 as an electrocatalyst for oxidation and, therefore, the determination of cholesterol. The highest value and the best reproducibility of analytical signal are reached at a CoCl2 concentration of 25 mM and a scan rate of 100 mV.s(-1). The linear region of the calibration curve ``current-cholesterol concentration{''} obtained using a developed method in a solution of 0.1 M LiClO4 and 25 mM CoCl2 in acetonitrile is 25-200 mu M (R-2 = 0.971) with a detection limit of 2 mu M. (C) 2016 Elsevier B.V. All rights reserved.

Author keywords:

Cholesterol; Electrochemical oxidation; Electrocatalysis; Cyclic voltammetry; Platinum electrode; Rotating disk electrode; Cobalt (II) chloride; Amperometric determination INDIRECT ELECTROCHEMICAL OXIDATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; ROTATING-DISK ELECTRODE; NANOPARTICLES; BIOSENSOR; SENSOR; ACETONITRILE; OXIDASE; GLUCOSE

DOI:

10.1016/j.jelechem.2016.04.029

Web of Science ID:

ISI:000377321400012

Соавторы в МНС:

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Другие поля

Поле	Значение
Month	JUL 1
Publisher	ELSEVIER SCIENCE SA
Address	PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Language	English
EISSN	1873-2569
Keywords-Plus	INDIRECT ELECTROCHEMICAL OXIDATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; ROTATING-DISK ELECTRODE; NANOPARTICLES; BIOSENSOR; SENSOR; ACETONITRILE; OXIDASE; GLUCOSE
Research-Areas	Chemistry; Electrochemistry
Web-of-Science-Categories	Chemistry, Analytical; Electrochemistry
Author-Email	a.v.ohohonin@urfu.ru
ResearcherID-Numbers	Kozitsina, Alisa/S-6317-2017 Okhokhonin, Andrei/H-2548-2012
ORCID-Numbers	Okhokhonin, Andrei/0000-0003-2922-7623
Funding-Acknowledgement	RFBR (Russian Foundation for Basic Research) {[}14-03-01017 A]
Funding-Text	This work was supported by RFBR (Russian Foundation for Basic Research, Grant 14-03-01017 A).
Number-of-Cited-References	30
Usage-Count-Last-180-days	2
Usage-Count-Since-2013	18
Journal-ISO	J. Electroanal. Chem.
Doc-Delivery-Number	DN8IC