Morphological features of the transgenic tobacco plant shoot expressing the 3-hydroxy-3-methylglutagyl-CoA reductase (HMG1) gene in the direct and reverse orientations towards the promoter / Ermoshin A.A., Kiseleva I.S., Bortsova S.A., Sanaeva Y.V., Alekseeva V.V. // Russian Journal of Developmental Biology. - 2016. - V. 47, l. 4. - P. 216-222.

ISSN:
10623604
Type:
Article
Abstract:
3-Hydroxy-3-methylglutaryl-CoA reductase (HMG1) catalyzes the formation of mevalonic acid, the key intermediate of the cytosolic isoprenoid synthesis pathway. The parameters of stem and leaf growth were studied in the transgenic tobacco plants that express the HMG1 gene in both sense and antisense orientations towards the constitutive promoter. The transgenic plant height did not significantly differ from that of the control plants, though the plants carrying the sense copy of the HMG1 gene were considerably taller than plants that carried the antisense gene copy. Plants carrying an extra copy of the HMG1 gene were also characterized by increased leaf area. The number of mesophyll cells calculated per square unit of transgenic plants leaves was smaller than in the control plant leaves, though their volume was not considerably changed in any of the variants, suggesting changes in the cell packing density in leaves. © 2016, Pleiades Publishing, Inc.
Author keywords:
HMG1 gene; leaf area; mesophyll; shoot height; transgenic tobacco plants
Index keywords:
нет данных
DOI:
10.1134/S1062360416040044
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979742645&doi=10.1134%2fS1062360416040044&partnerID=40&md5=50edf139b86e11cfba969548ca247199
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Link https://www.scopus.com/inward/record.uri?eid=2-s2.0-84979742645&doi=10.1134%2fS1062360416040044&partnerID=40&md5=50edf139b86e11cfba969548ca247199
Affiliations Yeltsin Ural Federal University, Ekaterinburg, Russian Federation; Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Russian Federation
Author Keywords HMG1 gene; leaf area; mesophyll; shoot height; transgenic tobacco plants
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Correspondence Address Bortsova, S.A.; Yeltsin Ural Federal UniversityRussian Federation; email: bos_o@mail.ru
Publisher Maik Nauka Publishing / Springer SBM
Language of Original Document English
Abbreviated Source Title Russ. J. Dev. Biol.
Source Scopus